论文部分内容阅读
对从普通小麦与中间偃麦草杂交后代中选育的一个抗白粉病新品系(AF-1)进行了形态学、细胞学和原位杂交(GISH)鉴定。结果表明:AF-1具有与小麦亲本相似的农艺性状,根尖细胞染色体数目为2n=42,花粉母细胞减数分裂中期(PMC MI)染色体构型为2n=21Ⅱ,且未见其他结构变异,细胞学上十分稳定。GISH结果表明,AF-1为中间偃麦草与普通小麦的一个小片段易位系,易位点位于一对染色体臂的中部偏着丝粒的位置。结合抗病调查结果,推断该片段上携带了一个来自偃麦草的抗白粉病基因。该片段可能是在杂种早期世代通过部分同源染色体配对或者单价染色体错分裂而形成的。
Morphological, cytological and in situ hybridization (GISH) identification of a new powdery mildew resistant line (AF-1) selected from the cross of common wheat and Thinopyrum intermedium was carried out. The results showed that AF-1 had agronomic traits similar to those of wheat parents. The chromosome number of root tip cells was 2n = 42 and the chromosome configuration of pollen mother cells was 2n = 21Ⅱ in the metaphase of meiosis, and no other structural variation , Cytologically very stable. The results of GISH indicated that AF-1 was a small fragment translocation line between Thinopyrum intermedium and common wheat, and the translocation site was located at the centromere of a pair of chromosome arms. Based on the results of the disease resistance survey, it was concluded that this fragment carries a powdery mildew resistance gene from Elymus. This fragment may have been formed by partial homologous chromosome pairing or monovalent chromosome misdiagnosis in early hybrid generations.