Magnetic nanoparticles (MNPs) have become a research hotspot and widely used in the biomedical field in recent decades due to their unique magnetic properties. This minireview summarizes the specific gene transfection of magnetic particles (magnetofection) during eversy dynamic process of gene delivery(gene binding, cellular uptake, endosomal escape, intracellular trafficking and in vivo targeting).Meanwhile, the synergistic biomedical application of magnetofection and the effects of MNPs have also been discussed, including magnetic resonance imaging (MRI), magnetic mediated hyperthermia (MMH),Fenton reaction and autophagy. Finally, the clinical prospect of magnetofection was briefly expected.