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为探讨运动对心脏心钠素受体基因表达的影响 ,建立不同强度运动训练动物模型 ,采用免疫荧光组织化学法、原位杂交、激光共聚焦扫描和计算机图像分析技术 ,观察心脏心钠素受体的分布和运动对心脏心钠素受体基因表达的影响 ,结果表明 ,心钠素受体主要分布在心内膜、心肌纤维的肌膜、心肌纤维间的结缔组织和冠状动脉分支的血管壁。中等和大强度运动使心脏A型心钠素受体基因表达上调和C型心钠素受体基因表达下调 ,力竭运动使A型心钠素受体基因表达下调和C型心钠素受体基因表达上调。
In order to investigate the effect of exercise on the expression of atrial natriuretic peptide receptor (ANP) in heart, animal models of exercise training with different intensities were established. Immunofluorescence histochemistry, in situ hybridization, confocal laser scanning microscopy and computer image analysis were used to observe the changes of atrial natriuretic peptide Body distribution and exercise on the expression of atrial natriuretic peptide receptor, the results showed that atrial natriuretic peptide receptors are mainly distributed in the endocardium, myocardial muscle fibers, connective tissue between myocardial fibers and coronary artery branches of the vessel wall . Moderate and high-intensity exercise increased the expression of atrial natriuretic peptide receptor gene and the type-C atrial natriuretic peptide receptor gene in the heart. Exhausted exercise reduced the expression of the type-A atrial natriuretic peptide receptor gene and the type-C atrial natriuretic peptide Up-regulated gene expression.