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DpCPV对8种鳞翅目昆虫感染试验结果表明,棉铃虫最敏感,感染率达97.0%。DpCPV对棉铃虫的毒力LC50=7.65×104CPB/ml,浓度与感染死亡率相关性及回归方程均达到显著性。HaDpCPV与原毒种DpCPV的形态特征相同;棉铃虫可用人工半合成饲料配方2在小塑料盒内单虫饲养,幼虫成活率达98%以上、羽化率达90%,平均蛹重0.4006g,平均产卵量797.7粒,卵孵化率达98.2%;HaDpCPV对第一代4~5龄马尾松毛虫的毒力较强,LC50=1.14×104CPB/ml,与原毒种相比,毒力没有下降。用棉铃虫来生产DpCPV,采用23~26℃、3龄虫、使用浓度105CPB/ml以上,是比较理想的组合。将病毒喷在人工饲料表面喂虫,可获得较高的病毒产量,平均每条虫可产8.13亿CPB,与用原宿主生产的产量相接近。用HaDpCPV防治第一、二代4~5龄马尾松毛虫,防治效果达60.2%~84.0%。
The results of DpCPV infection test on eight Lepidoptera insects showed that the bollworm was the most sensitive and the infection rate was 97.0%. The toxicity of DpCPV against Helicoverpa armigera (LC50) was 7.65 × 104 CPB / ml, the correlation between concentration and infection mortality and the regression equation reached significance. The morphological characteristics of HaDpCPV and the original strain DpCPV were the same. The bollworm was fed on the single plastic insects in a small plastic box. The survival rate of the larvae reached over 98% with the emergence rate of 90% and the average pupal weight of 0. 4006g, the average number of eggs laid was 797.7, egg hatching rate was 98.2%; Ha DpCPV virulence of the first generation of 4 ~ 5 age-dependent Dendrolimus punctatus, LC50 = 1.14 × 104CPB / ml, Compared with the original poison, virulence did not decline. It is an ideal combination to produce DpCPV by using the cotton bollworm, using 23 ~ 26 ℃ and 3rd instar larvae with the concentration above 105CPB / ml. The virus sprayed on artificial feed surface feeding insects, access to higher virus production, the average per worm can produce 813 million CPB, with the production of the original host close. With Ha DpCPV control first and second generation 4 to 5-year-old Dendrolimus punctatus, control effect of 60.2% to 84.0%.