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铜绿假单胞菌WJ-1是从蒙古林油田油水样中分离得到的一株能够以原油为唯一碳源,通过合成某些代谢产物增加其环境竞争力和存活率的烃降解菌.用氯仿从WJ-1LB培养液中萃取绿脓素,气相色谱-质谱法(Gas chromato-graph-Mass Spectrometry,GC-MS)分析得该物质为1-羟基吩嗪.通过在原油培养基中加入和未加入0.2 g/L绿脓素,培养32 d来研究绿脓素对烃降解菌群T2的群落结构和原油降解能力的影响.变性梯度凝胶电泳(Denaturing Gradient Gel Electrophoresis,DGGE)分析结果表明:在32 d的原油降解中,含有绿脓素的培养液中T2菌群多样性减少.气相色谱和棒薄层层析分析得:含有绿脓素的培养液中原油的芳香族烃等非烃类和正构烷烃中长链组分的降解率比未加入绿脓素的要低.超氧化物歧化酶(SOD)和过氧化氢酶(catalase)活性检测结果表明:不同菌体的过氧化氢酶和超氧化物歧化酶含量与活性不同,引起对绿脓素抗性的差异.可见绿脓素对烃降解菌群的结构和原油降解能力有负面影响.
Pseudomonas aeruginosa WJ-1 is a hydrocarbon-degrading bacterium isolated from the oil-water samples in the Mengulin oilfield and capable of using crude oil as the sole carbon source to increase its environmental competitiveness and survival through the synthesis of certain metabolites. Chloroform was used to extract pyocyanin from WJ-1LB medium and analyzed by gas chromato-graph-mass spectrometry (GC-MS) to obtain 1-hydroxyphenazine. The effect of pyocyanin on the community structure of hydrocarbon-degrading bacteria T2 and the degradation ability of crude oil were studied without addition of 0.2 g / L pyocyanin for 32 days.The results of Denaturing Gradient Gel Electrophoresis (DGGE) : During the 32-day degradation of crude oil, the diversity of T2 flora in the culture medium containing pyocyanin was reduced.Gas chromatography and thin-layer chromatography analysis showed that the aromatic hydrocarbons in the crude oil in the medium containing pyocyanin were not The degradation rate of long-chain components in hydrocarbons and normal paraffins was lower than that without adding pyocyanin.The results of SOD and catalase activity showed that: Hydrogenase and superoxide dismutase content and activity caused by Differences pyocyanin resistance seen pyocyanin hydrocarbon structure have a negative impact on the ability to degrade and the degradation of crude flora.