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A蛋白与辣根过氧化物酶交联,可为酶联免疫试验提供大量的酶结合物,对推广ELISA的广泛应用很有意义。我们参照丁宗武等的报导进行了辣根过氧化物酶与A蛋白的酶联试验,简报如下。 材料和方法 辣根过氧化物酶与A蛋白的交联: A蛋白购自上海生物制品研究所,每安瓶6mg冻干纯品,批号81—9—1500。与酶交联前将6mgA蛋白溶于1ml 0.015M pH9.6的Na_2CO_3/NaHCO_3缓冲液中,平衡24小时。
Protein A cross-linked with horseradish peroxidase (HRP) can provide a large number of enzyme conjugates for enzyme-linked immunoassay, which is of great significance to the widespread application of ELISA. We refer to the Ding Zongwu et al reported horseradish peroxidase and protein A enzyme-linked test, the briefing is as follows. MATERIALS AND METHODS Crosslinking of horseradish peroxidase to protein A: Protein A was purchased from Shanghai Institute of Biological Products, with 6 mg of lyophilized pure product per ampoule, lot number 81-9-1500. 6mgA protein was dissolved in 1ml 0.015M Na 2 CO 3 / NaHCO 3 buffer pH9.6 before cross-linking with enzyme for 24 hours.