The widespread microbialites deposition that followed the End-Permian mass extinction in the Tethyan realm have been intensively studied because of the evidence they provide on the nature of this crisis and its aftermath. However, the age of the microbialite event remains controversial. New conodont collection across the Permian–Triassic(P–T) transition from Dajiang(Guizhou Province, South China) in this study enable us to discriminate four conodont zones, in ascending order, they are: Hindeodus parvus zone, Isarcicella lobata zone, Isarcicella isarcica zone and Hindeodus sosioensis zone. The age of microbialite in the P–T transition at the Dajiang Section is considered to be within the Hindeodus parvus zone and thus to clearly post-date the main extinction crisis. Reviewing the age of onset of microbialites throughout the Tethyan regions reveals two different ages: a Hindeodus changxingensis zone age is dominant in south-western and westernmost Tethys, whilst most other regions show microbialite deposition began in the Hindeodus parvus zone. Our investigation also indicates that two conodont changes occur at this time: an increase of hindeodid species immediately following a sequence boundary and the mass extinction, and a phase of extinction losses in the earliest Triassic Isarcicella isarcica zone during highstand development.