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目的:观察miR-3074-5p及其靶基因n p27在子痫前期胎盘组织中的表达情况，初步探讨miR-3074-5p/n p27分子途径在子痫前期病理过程中的作用。n 方法:收集2017年9月至2018年3月期间在天津医科大学第二医院产科行剖宫产手术的子痫前期患者（子痫前期组，16例）和相同孕周非子痫前期的剖宫产产妇（对照组，9例）的胎盘组织，通过实时定量PCR、免疫组织化学（immunohistochemistry，IHC）和Western blotting检测，比较两组胎盘组织中miR-3074-5p以及p27、细胞周期蛋白D1（cyclin D1，CCND1）和细胞周期蛋白依赖性激酶2（cyclin dependent kinase 2，CDK2）等蛋白的表达水平。应用双荧光素酶基因报告系统，验证miR-3074-5p对n p27 mRNA的靶向抑制作用；通过RNA干扰技术，敲低人绒毛外滋养细胞系HTR-8/SVneo中p27蛋白的表达水平，观察p27表达下调对细胞生理活性的影响。n 结果:与对照组相比，子痫前期组胎盘组织中miR-3074-5p（n P=0.034）与CCND1蛋白的表达量都显著下调（n P=0.031），而p27蛋白的表达量则显著上调（n P=0.010）；IHC结果显示，p27和CCND1蛋白主要表达于合体滋养层细胞中。双荧光素酶报告系统检测结果证实，miR-3074-5p能与n p27 mRNA的3’UTR序列结合而抑制其表达；敲低HTR-8/SVneo细胞中n p27的表达水平后，细胞的增殖（n P=0.014）和侵袭（n P=0.045）活性都显著增强。n 结论:miR-3074-5p可能通过直接靶向抑制n p27的表达而参与调控人绒毛外滋养层细胞的生理活性，进而影响胎盘发育及功能；胎盘组织中miR-3074-5p的异常低表达可能通过诱导n p27的表达而参与子痫前期的病理过程。n “,”Objective:To detect the expression levels of miR-3074-5p and its target gene n p27 in the placental tissues of pre-eclampsia (PE) patients, and to preliminarily explore the association of miR-3074-5p/p27 pathway in the pathogenesis of PE.n Methods:From September 2017 to March 2018, 16 pregnant women with PE (PE group) and 9 normal pregnant women (control group) were enrolled in Obstetrics Department in the Second Hospital of Tianjin Medical University. The placental expression levels of miR-3074-5p, n p27, cyclin D1 (CCND1) and cyclin dependent kinase 2 (CDK2) were determined by quantitative PCR (qPCR), Western blotting and immunohistochemistry (IHC) analyses. The dual-luciferase reporter assay was applied to authenticate the directly targeted effect of miR-3074-5p on expression of n p27 mRNA. The expression of n p27 in the human extravillous trophoblast cell line HTR-8/SVneo was down-regulated by its specific shRNA, and the effects of down-regulated n p27 expression on physiological activities of HTR-8/SVneo cells were detected.n Results:Compared with control group, the expression levels of miR-3074-5p (n P=0.034) and CCND1 (n P=0.031) protein in the placenta of PE patients were significantly decreased, whereas the placental p27 (n P=0.010) protein expression was significantly increased in PE group. IHC analysis showed that, the signals of p27 and CCND1 proteins were dominantly detected in syncytiotrophoblast. The results of dual-luciferase reporter assay confirmed that miR-3074-5p could inhibit the expression of n p27 by directly bound to the 3\'UTR of n p27 mRNA. After the n p27 expression was down-regulated, the proliferative and invasive activities of HTR-8/SVneo cells were significantly enhanced (n P=0.014, n P=0.045).n Conclusion:miR-3074-5p might participate in the regulation of physiological activities of extravillous trophoblast cells by its targeted inhibition on n p27 expression, and the abnormally decreased miR-3074-5p in trophoblast cells might be associated with the pathogenesis of PE by promoting the expression of n p27.n