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目的 探索地佐辛对坐骨神经慢性压迫性疼痛(CCI)大鼠脊髓胶质纤维酸性蛋白(GFAP)的影响及对坐骨神经的保护作用.方法 按照体重将SD大鼠随机为4组,每组8只:假手术组、模型组和低、高2个剂量实验组(地佐辛,2.5,10 mg·kg-1).于手术后即刻至术后7d,各组每天腹腔注射药物1次,假手术组和模型组给予等体积的0.9％ NaCl.于给药7d后,电镜下观察各组坐骨神经的病理形态学变化,以免疫荧光组织化学法观测各组脊髓GFAP表达.结果 地佐辛连续给药7d后,低剂量实验组大鼠的坐骨神经纤维肿胀,鞘细胞数目减少,部分髓鞘溶解;高剂量实验组大鼠有少数正常髓鞘,较模型组神经纤维肿胀减轻,但较假手术组鞘细胞减少,部分髓鞘中可见轴突.大鼠脊髓中GFAP的表达水平:假手术组、模型组、低、高2个剂量实验组分别为1.03±0.28,2.08 ±0.55,1.61 ±0.14,1.25 ±0.45.与假手术组比较,模型组差异有统计学意义(P＜0.001);与模型组比较,低、高2个剂量实验组差异均有统计学意义(P ＜0.05,P＜0.01).结论 地佐辛对神经病理性疼痛的抑制作用,可能与下调脊髓GFAP的表达、影响星形胶质细胞的活化和减轻坐骨神经水肿有密切关联.“,”Objective To investigate the influence of dezocine on spinal cord glial fibrillary acidic protein (GFAP) in rats with chronic constriction injury (CCI) of sciatic nerve as well as its protective effect on sciatic nerve.Methods Thirty two SD rats were randomly divided into sham-operation group,model group,experimental-L,-H groups (with 2.5,10 mg · kg-1 dezocine),eight SD rats in each group.The SD rats in experimental groups were treated with dezocine by intraperitoneal injection once every day from the beginning to seven days after operation;but the SD rats in sham-operation group and model group were treated with normal saline.Seven days after operation,the changes of pathological morphological of sciatic nerve in the four groups were detected.And the expression of GFAP in the four groups was detected by immunofluorescence histochemistry.Results After continuous injection of dezocine for 7 days,the sciatic nerve fibers in experimental-L group rats were swelling,the number of sheath cells decreased,and some of them were dissolved.In experimental-H group,there were a few normal myelin in rats,the swelling of nerve fibers is reduced compared with those in model group,but the number of sheath cells decreased compared with those in sham group,axons can be seen in some myelin sheaths.The expression of glial fibrillary acidic protein of spinal cord in sham-operation group,model group,experimental-L,-H groups were 1.03 ± 0.28,2.08 ±0.55,1.61 ±0.14,1.25 ±0.45.Compared with sham group,the difference in model group was significant (P ＜0.001);compared with model group,the difference in experimental-L,-H groups was significant(P ＜ 0.05,P ＜ 0.01).Conclusion Dezocine can inhibit the neuropathic pathologic pain.The mechanism maybe related to the decrease of the expression of GFAP in spinal cord,the activation of astrocytes,and the relief of the edema of sciatic nerve.