目的　追溯某教学医院神经外科ICU病房发生的一次铜绿色假单胞菌医院感染 ,探讨细菌分型方法。方法　从患者痰标本及周围环境中分离得到 7株铜绿色假单胞菌 ,应用生物、抗菌谱、血清和质粒图谱进行分型。结果　 7株铜绿色假单胞菌具有相同的血清型和质量粒谱型。结论　此次铜绿色假单胞菌医院感染来源于同一克隆菌株 ,铜绿色假单胞菌的 4种分型结果显示 ,质粒谱分型和血清分型的一致性较好 Objective To trace a Pseudomonas aeruginosa nosocomial infection in the neurosurgery ICU ward of a teaching hospital and discuss the method of bacterial typing. Methods Seven strains of Pseudomonas aeruginosa were isolated from the sputum of patients and their surroundings, and the biological, antibacterial, serum and plasmid profiles were used for typing. Results Seven strains of Pseudomonas aeruginosa had the same serotype and mass speciation. Conclusions The hospital infection of Pseudomonas aeruginosa comes from the same clonal strain. The four typing results of Pseudomonas aeruginosa show that the consistency of plasmid spectrum typing and serum typing is good