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为了解石棉及电焊烟尘相关肺癌的K-ras基因突变情况,并与非职业肺癌的K-ras基因突变特点比较,进一步探讨石棉及电焊烟尘的致癌机理。收集8例非职业肺癌组织、9例石棉相关肺癌组织及4例电焊烟尘相关肺癌组织,提取基因组DNA,经巢式聚合酶链反应(PCR)特异性扩增k-ras基因片段后,进行限制性片段长度多态性分析(RFLP)、单链构象多态性分析(SSCP)及DNA 直接测序分析。K-ras 基因突变百分比在非职业肺癌组为25% ,石棉相关肺癌组为44.4% ,电焊烟尘相关肺癌组为25% ;突变位点方面,非职业肺癌以12密码子为突变热点,石棉相关肺癌则相对集中在12及15密码子,电焊烟尘相关肺癌组则发现在13密码子有突变;碱基替换形式,非职业肺癌以G→T颠换为主,石棉相关肺癌及电焊烟尘相关肺癌以G→A转换为主。结果提示,石棉相关肺癌与非职业肺癌及矽尘相关肺癌有着不同的K-ras基因突变规律,提示三者的发病机理可能不同。由于样本量限制,电焊烟尘相关肺癌的K-ras基因突变暂时看不出特殊规律
In order to understand the mutation of K-ras gene in asbestos and welding fume-associated lung cancer, and to compare with the characteristics of K-ras gene mutation in non-occupational lung cancer, to further explore the carcinogenic mechanism of asbestos and welding fume and dust. Genomic DNA was extracted from 8 cases of non-occupational lung cancer, 9 cases of asbestos-related lung cancer and 4 cases of welding fume-associated lung cancer. The k-ras gene fragment was amplified by nested polymerase chain reaction (PCR) RFLP, single-strand conformation polymorphism (SSCP) analysis and DNA sequencing. The percentage of K-ras gene mutation was 25% in non-occupational lung cancer group, 44.4% in asbestos-related lung cancer group and 25% in welding dust-related lung cancer group. In terms of mutation sites, a non-occupational lung cancer with 12 codons as hotspot mutation and asbestos- Lung cancer is relatively concentrated in the 12 and 15 codons, the welding of dust-related lung cancer group found at codon 13 mutation; base replacement, non-professional lung cancer to G → T transversion, asbestos-related lung cancer and welding dust-related lung cancer To G → A conversion-based. The results suggest that asbestos-related lung cancer and non-occupational lung cancer and silica-related lung cancer have different K-ras gene mutations, suggesting that the pathogenesis of the three may be different. Due to sample size limitations, K-ras mutations in welding fumes associated with lung cancer temporarily do not show any specific pattern