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目的:探讨E6AP基因与HPV16、18蛋白表达的关系及其在宫颈癌发生、发展过程中的作用和意义。方法:在40例宫颈癌、10例CIN及10例正常宫颈组织中采用免疫组化检测宫颈癌组织中HPV-16、18型E6蛋白表达,RT-PCR技术检测E6AP基因的表达。结果:宫颈癌组织中HPV-16、18型E6蛋白的阳性表达明显高于CIN组织和正常宫颈组织,CIN组织中的阳性表达高于正常宫颈组织,差异均有统计学意义。E6蛋白阳性组织E6APmRNA的表达(0.0946±0.0715)明显高于阴性组织中的E6APmRNA表达(0.0062±0.0103),差异有统计学意义。E6AP基因在癌组织中表达高于CIN组织及正常宫颈组织(P<0.05),E6AP的表达水平随临床分期的进展而升高(P<0.05)。结论:E6AP基因的表达与高危型人乳头瘤病毒HPV16、18E6蛋白的表达具有密切的关系,参与了宫颈上皮癌变的发生、发展过程,与宫颈癌的发生、发展密切相关。
Objective: To investigate the relationship between E6AP gene and HPV16,18 protein expression and its role in the occurrence and development of cervical cancer. Methods: The expression of E6 protein in cervical cancer tissues was detected by immunohistochemistry in 40 cases of cervical cancer, 10 cases of CIN and 10 cases of normal cervical tissue. The expression of E6AP gene was detected by RT-PCR. Results: The positive expression of HPV16,18 and E6 in cervical cancer was significantly higher than that in CIN and normal cervical tissues, and the positive expression in CIN was higher than that in normal cervical tissues. The differences were statistically significant. The expression of E6AP mRNA in E6 positive tissues (0.0946 ± 0.0715) was significantly higher than that in negative tissues (0.0062 ± 0.0103), the difference was statistically significant. The expression of E6AP in cancer tissues was higher than that in CIN tissues and normal cervical tissues (P <0.05). The expression of E6AP increased with the clinical stage (P <0.05). CONCLUSION: The expression of E6AP gene is closely related to the expression of HPV16, HPV-18 and HPV-6 in high-risk human papillomavirus. It is involved in the carcinogenesis and progression of cervical carcinogenesis, which is closely related to the occurrence and development of cervical cancer.