牛磺酸作为新型营养强化剂添加在第二代母乳化奶粉中。作者应用高效液相色谱法对母乳化奶粉中的牛磺酸进行定量分析，样品经1％偏磷酸提取→钠柱分离→柱后OPA衍生→荧光检测器检测定量。操作简便、快速，提取完全，牛磺酸出峰时间8．738min，回收率99．5％，变异系数2．19％，最低检出量20ng，为一个较理想的定量方法。 Taurine as a new nutrition fortifier added to the second generation of milk powder. The author uses high performance liquid chromatography (HPLC) to quantitatively analyze taurine in breast milk powdered milk. The sample is extracted by 1% metaphosphoric acid → sodium column separation → OPA derivatization after column → fluorescence detector. The method is simple, rapid and complete. The taurine peak time 8.738min, the recovery rate 99.5%, the coefficient of variation 2.19%, the minimum detectable amount 20ng, is an ideal quantitative method.