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健康人血清中游离氨基酸的含量是恆定的,当前用微生物法和纸上层折法测定氨基酸是最灵敏的方法。本文详细介绍利用双向纸上层析的方法,测定人血清中的游离氨基酸的操作方法,并例举28个健康人血清进行试硷,在双向色层谱上得到明显的16个氨基酸的斑点。但在人血清中甘氨酸,苏氨酸和二羧氨基酸浓度变动很大,在同一个人在不同时间里,测定含
The content of free amino acids in healthy human serum is constant, and currently the most sensitive method for the determination of amino acids by microbial methods and paper laminates. This article describes in detail the method for the determination of free amino acids in human serum by two-way paper chromatography, and provides an example of a test of 28 healthy human serum samples to give clear 16 amino acid spots on the two-dimensional chromatography. However, in human serum glycine, threonine and dicarboxylamino acid concentrations vary widely in the same person at different times, the determination of containing