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目的探讨顺铂联合胞嘧啶脱氨酶(CD)自杀基因对直肠腺癌细胞8348的杀伤作用。方法以绿色荧光蛋白(GFP)基因作为报告基因,观察在顺铂作用下GFP基因对8348细胞的转染效率;用克隆形成试验观察顺铂对CD基因转染效率的影响;同时用四甲基偶氮唑盐(MTT)试验检测顺铂联合CD/5-氟胞嘧啶(5-FC)对8348细胞的杀伤作用。结果顺铂提高质粒对8348细胞的转染效率26倍。对腺癌细胞的杀伤效率:单纯转染CD基因组为14.9%;IC50的氟尿嘧啶(5-FU)联合CD基因组为54.9%;IC50的顺铂联合CD基因组为88.5%,与其他两组相比,对癌细胞杀伤效率明显增强(P<0.01)。结论顺铂联合CD自杀基因能更有效地杀伤直肠腺癌细胞,可成为治疗直肠癌术后复发及转移的一种有效的方法。
Objective To investigate the killing effect of cisplatin combined with cytosine deaminase (CD) suicide gene on rectal adenocarcinoma cell line 8348. Methods GFP gene was used as a reporter gene to observe the transfection efficiency of GFP gene on 8348 cells under the action of cisplatin. The effect of cisplatin on the transfection efficiency of CD gene was observed by clonogenic assay. At the same time, The cytotoxicity of cisplatin combined with CD / 5-fluorocytosine (5-FC) on 8348 cells was detected by MTT assay. Results Cisplatin increased plasmid transfection efficiency to 8348 cells by 26-fold. The killing rate of adenocarcinoma cells was 14.9% in CD group; 54.9% in IC50 with fluorouracil (5-FU) combined with CD genotype; 88.5% in IC50 with cisplatin and CD genotype. Compared with the other two groups, On the cancer cell killing efficiency was significantly enhanced (P <0.01). Conclusion Cisplatin combined with CD suicide gene can kill rectal adenocarcinoma cells more effectively and may become an effective method for the treatment of rectal cancer recurrence and metastasis.