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目的:研究肝硬化患者红细胞CD44数量与血清透明质酸含量变化的关系。方法:将戊二醛固定的2% 红细胞悬液定量“液相包被”到V型板中,依次加入鼠抗人CD44单克隆抗体、碱性磷酸酶标记的羊抗鼠第二抗体,洗板后加底物显色,移显色液于比色板中,测定其A405吸光值。本文对111例肝硬化病人的红细胞CD44进行测定,同时采用AU600全自动生化仪检测其胆碱酯酶(CHE),采用ACL200检测凝血酶原活动度(PTA),采用ELISA定量试剂盒检测血清透明质酸含量。结果:肝硬化患者红细胞CD44数量表达都显著低于正常对照,并且重度肝硬化患者的红细胞CD44的数量变化显著低于轻度肝硬化患者(t=-4. 55,P=5. 76×10-6 ),肝硬化患者红细胞CD44变化与血清HA含量的变化关系密切(P<0. 01),同时与CHE和PTA的变化密切相关(P<0 .05)。结论:肝硬化患者的红细胞CD44数量变化与其病情发展关系密切。
Objective: To study the relationship between the number of erythrocyte CD44 and the change of serum hyaluronic acid in cirrhotic patients. Methods: The glutaraldehyde-fixed 2% erythrocyte suspension was quantitatively “liquid-phase coated” into a V-shaped plate, followed by the addition of a mouse anti-human CD44 monoclonal antibody and alkaline phosphatase-labeled goat anti-mouse secondary antibody After the plate plus substrate color, color development solution in the color palette, measured A405 absorbance value. In this paper, 111 cases of cirrhosis of patients with erythrocyte CD44 determination, while using AU600 automatic biochemical analyzer to detect its cholinesterase (CHE), using ACL200 prothrombin activity (PTA), using ELISA quantitative kit for detection of serum transparent Hyaluronic acid content. Results: The expression of CD44 in erythrocytes in patients with cirrhosis was significantly lower than that in normal controls, and the number of CD44 in erythrocytes in patients with severe cirrhosis was significantly lower than that in patients with mild cirrhosis (t = -4.55, P = 5.76 × 10 -6). The changes of erythrocyte CD44 in cirrhotic patients were closely related to the changes of serum HA levels (P <0.01), and were closely related to the changes of CHE and PTA (P <0.05). Conclusion: The number of erythrocyte CD44 in patients with cirrhosis is closely related to the progression of the disease.