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将内吞进入细胞内部的金纳米颗粒作为表面增强拉曼散射光谱的活性增强基底,通过共聚焦显微拉曼光谱仪测试单个人乳腺癌细胞(MCF-7)的常规拉曼光谱和表面增强拉曼光谱,并进行了初步峰位归属分析及不同拉曼峰强度柱状图对比分析。实验结果表明,内吞金纳米颗粒的单个MCF-7细胞的表面增强拉曼光谱相比于常规拉曼光谱包含更多的细胞生化物质拉曼信号,并且谱线强度有显著增强。由于细胞中各种生物分子在结构和含量上的变化可以反映细胞代谢及病理变化,因此,以具有高灵敏度的金纳米颗粒作为生物分子探针可能成为探测细胞内部组分信息的一种有效的方法,基于内吞金纳米颗粒的单个MCF-7细胞SERS技术在乳腺癌的诊断和治疗方面具有广阔的应用前景。
The gold nanoparticles endocytosed into the cells were used as the substrate for the activity enhancement of surface enhanced Raman scattering spectroscopy and the conventional Raman spectra and surface enhancement of single human breast cancer cells (MCF-7) were tested by confocal Raman spectroscopy Man spectroscopy, and the initial peak position attribution analysis and different Raman peak intensity histogram comparative analysis. The experimental results show that the surface enhanced Raman spectra of single MCF-7 cells with swallowed gold nanoparticles contain more Raman signals than those of conventional Raman spectra and the intensity of the spectral line is significantly enhanced. Because the changes in the structure and content of various biological molecules in cells can reflect the changes of cell metabolism and pathology, using gold nanoparticles with high sensitivity as biomolecular probes may be an effective method for detecting the information of intracellular components Method, a single MCF-7 cell SERS technology based on endocytosed gold nanoparticles has broad application prospects in the diagnosis and treatment of breast cancer.