论文部分内容阅读
目的:探讨联合应用rhTNF和环磷酰胺治疗胶质瘤大鼠的效果及其可能机制。方法:制备胶质瘤大鼠模型,动态监测经颈总动脉给予rhTNF后的血肿瘤屏障开放程度。给予rhTNF 120 min再给予CTX后,ELISA法测定胶质瘤组织内CTX的含量。观察分别给予rhTNF、CTX和rhTNF(120 min)+CTX的胶质瘤大鼠的生存时间。结果:给胶质瘤大鼠注射rhTNF后,血肿瘤屏障开放程度增加,于120 min达最大后开始减小。给予rhTNF 120 min再给予CTX后,胶质瘤组织内CTX含量明显高于单独给予CTX组。与其他组相比,rhTNF(120 min)+CTX能明显延长胶质瘤大鼠的生存时间。结论:胶质瘤大鼠给予rhTNF 120 min时血肿瘤屏障开放程度最大,此时进入胶质瘤组织的CTX含量最多。
Objective: To investigate the effect of rhTNF combined with cyclophosphamide on glioma in rats and its possible mechanism. Methods: The rat model of glioma was prepared and the degree of openness of hematological tumor barrier was dynamically monitored by rhTNF. After CTX was given to rhTNF for 120 min, the content of CTX in glioma tissues was determined by ELISA. The survival of glioma rats were observed with rhTNF, CTX and rhTNF (120 min) + CTX respectively. Results: After the rhTNF was injected into glioma rats, the degree of openness of hematopoietic tumor barrier increased and reached the peak at 120 min and then began to decrease. After CTX was given to rhTNF for 120 min and then to CTX, the content of CTX in glioma tissue was significantly higher than that of CTX alone. Compared with other groups, rhTNF (120 min) + CTX significantly prolonged the survival time of glioma rats. CONCLUSION: The hematopoietic tumor barrier is most open to glioma rats after being given rhTNF for 120 min. The CTX content in glioma tissue is the highest at this time.