目的 研究和评价端粒酶逆转录酶反义核酸对K562端粒酶活性的影响。方法 用PCR-ELISA的方法,检测K562细胞经过反义核酸处理后端粒酶活性的变化;用RT-PCR的方法检测相应的mRNA的水平。结果 K562细胞经反义核酸处理48h以后,端粒酶逆转录酶的mRNA水平明显降低,端粒酶的活性受到了显著的抑制。结论 端粒酶逆转录酶的反义核酸在翻译水平能显著地抑制端粒酶的活性;同时,也证明了端粒酶逆转录酶与端粒酶活性之间的关系是密切相关的。 Objective To study and evaluate the effect of telomerase reverse transcriptase antisense nucleic acid on telomerase activity of K562. Methods The changes of telomerase activity in K562 cells treated with antisense oligonucleotide were detected by PCR-ELISA. The mRNA level of the corresponding mRNA was detected by RT-PCR. Results After treated with antisense oligonucleotide for 48h, the mRNA level of telomerase reverse transcriptase was significantly decreased and the activity of telomerase was significantly inhibited. CONCLUSION: Antisense nucleic acid of telomerase reverse transcriptase can significantly inhibit the activity of telomerase at the level of translation. At the same time, it is also demonstrated that the relationship between telomerase reverse transcriptase and telomerase activity is closely related.