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目的探讨阿魏酸乙酯(EF)对胃癌SGC-7901细胞增殖及凋亡的影响。方法体外培养人胃癌SGC-7901细胞,采用不同浓度的EF作用SGC-7901细胞,设置5个复孔,作用24、36、48 h,MTT法检测细胞的增殖情况;用0和40μg/m L EF分别作用SGC-7901细胞24 h,DAPI染色法观察SGC-7901细胞形态学变化;另外,采用不同浓度的EF作用SGC-7901细胞24、36和48 h,Annexin V-FITC/PI法和流式细胞仪检测细胞凋亡情况。结果 40、80、120、160和200μg/m L浓度的EF分别作用SGC-7901细胞24、36及48 h,其对SGC-7901细胞的IC50分别为158.86、142.48和128.07μg/m L;40μg/m L的EF作用SGC-7901细胞12 h,经DAPI染色,SGC-7901细胞的细胞核固缩,出现凋亡小体;经Annexin V-FITC/PI法检测,EF对SGC-7901细胞的凋亡有明显的诱导作用,并且随着EF作用浓度和时间的增加,凋亡率增加。结论 EF能有效抑制SGC-7901细胞增殖,并能诱导SGC-7901细胞凋亡,两种作用均呈浓度和时间依赖性。
Objective To investigate the effect of ethyl ferulate (EF) on the proliferation and apoptosis of gastric cancer cell line SGC-7901. Methods Human gastric cancer SGC-7901 cells were cultured in vitro. SGC-7901 cells were treated with different concentrations of EF. Five replicate wells were established for 24, 36 and 48 hours. MTT assay was used to detect the proliferation of SGC-7901 cells. SGC-7901 cells were treated with different concentrations of EF for 24, 36 and 48 h, Annexin V-FITC / PI and flow cytometry (FCM) were used to observe the morphological changes of SGC- Cytometry to detect apoptosis. Results The IC50 values of SGC-7901 cells were 158.86, 142.48 and 128.07 μg / mL respectively at 40, 80, 120, 160 and 200 μg / mL concentrations of EF for 24, 36 and 48 h, respectively. The apoptosis of SGC-7901 cells was observed by Annexin V-FITC / PI assay after being stained with DAPI for 12 h. There was a significant induction of apoptosis, and with the EF concentration and time increases, the rate of apoptosis increased. Conclusion EF can effectively inhibit the proliferation of SGC-7901 cells and induce the apoptosis of SGC-7901 cells in a concentration-dependent and time-dependent manner.