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目的评价银杏酮酯(EGb_(50))对大鼠坐骨神经损伤后神经再生的影响及其量效关系。方法建立大鼠坐骨神经损伤模型,将雄性SD大鼠96只随机分为4组：损伤对照组、银杏酮酯高剂量组(200mg·kg~(-1)·d~(-1))、银杏酮酯中剂量组(100mg·kg~(-1)·d~(-1))、银杏酮酯低剂量组(50mg·kg~(-1)·d~(-1))。分别于术后2、4、6、8周用电生理学、组织学和功能测定评估坐骨神经再生和功能恢复情况。结果术后坐骨神经功能指数(SFI)、小腿三头肌肌张力、运动神经传导速度、小腿三头肌肌细胞截面积恢复率及有髓神经纤维通过率在各时间点上银杏酮酯各剂量组均优于对照组(P＜0．01)。除坐骨神经功能指数外,其它各项指标银杏酮酯高剂量组均优于中低剂量组(P＜0．01,P＜0．05)。结论银杏酮酯可以促进损伤神经的再生,且对神经再生的促进作用存在一定的量效关系。
Objective To evaluate the effect of ginkgo biloba (EGb_(50)) on nerve regeneration after sciatic nerve injury in rats and its dose-effect relationship. Methods A rat sciatic nerve injury model was established. Ninety-six male Sprague-Dawley rats were randomly divided into 4 groups: injury control group, high dose group of ginkgo biloba ketone (200 mg·kg -1 ·d -1) and ginkgo The keto ester medium dose group (100 mg·kg -1 ·d -1) and the low-dose ginkgo ketone ester group (50 mg·kg -1 ·d -1). The sciatic nerve regeneration and functional recovery were evaluated by electrophysiological, histological and functional measurements at 2, 4, 6 and 8 weeks postoperatively. RESULTS Postoperative sciatic nerve function index (SFI), triceps brachii muscle tension, motor nerve conduction velocity, recovery rate of cross-sectional area of myocytes of the triceps brachii muscle, and myelinated nerve fiber passage rate at each time point. All were better than the control group (P<0.01). Except the sciatic nerve function index, all other indexes of high dose of gingko ketone ester group were superior to medium and low dose group (P<0.01, P<0.05). Conclusion Ginkgo Biloba extract can promote the regeneration of injured nerves, and there is a certain dose-effect relationship for the promotion of nerve regeneration.