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目的研究不同浓度安氟醚（Ｅｎｆ）对常温或低温下心肌能量代谢的影响。方法２４只家兔的离体心脏， Ｌａｎｇｅｎｄｏｒｆｆ装置平衡灌注 １５ｍｉｎ后，随机分为 ４组（ ｎ＝ ６）， Ａ组：常温 １． １％ Ｅｎｆ的Ｋ－Ｈ液灌注； Ｂ组：常温 ２． ２％ Ｅｎｆ的 Ｋ－Ｈ液灌注； Ｃ组：低温 １． １％ Ｅｎｆ的 Ｋ－Ｈ液灌注； Ｄ组：低温２． ２％ Ｅｎｆ的 Ｋ－Ｈ液灌注。常温组温度为 ３７℃，低温组温度为 ３０℃。灌注 ３０ｍｉｎ后，取心失部心肌组织测定腺苷酸含量、ＡＴＰ酶活性。结果常温或低温下， ２． ２％ Ｅｎｆ组 ＡＴＰ含量明显高于 １． １％ Ｅｎｆ组，Ｎａ＋－Ｋ＋ＡＴＰａｓｅ活性明显低于１．１％ Ｅｎｆ组（Ｐ＜０．０５或０．０１）；而低温下，２．２％ Ｅｎｆ组ＡＤＰ含量及ＥＣ明显高于１．１％ Ｅｎｆ组，Ｃａ２＋ＡＴＰａｓｅ活性明显低于１．１％ Ｅｎｆ组（Ｐ＜０．０５或０．０１）。结论 Ｅｎｆ通过抑制心肌细胞膜ＡＴＰａｓｅ活性导致心脏电生理受到抑制，低温可能加重安氟醚的心肌抑制作用。
Objective To study the effects of different concentrations of enf on myocardial energy metabolism at normal temperature and hypothermia. Methods Twenty-four rabbits were randomly divided into 4 groups (n = 6), group A: normal temperature 1, after the Langendorff apparatus was perfused for 15 minutes. 1% Enf K-H liquid perfusion; Group B: room temperature 2. 2% Enf K-H fluid perfusion; Group C: low temperature 1. 1% Enf K-H fluid perfusion; D group: low temperature 2. 2% Enf K-H fluid perfusion. Normal temperature group temperature is 37 ℃, low temperature group temperature is 30 ℃. After perfusion for 30min, the myocardial tissues were removed for determination of adenosine content and ATPase activity. Results at room temperature or low temperature, 2. ATP content in 2% Enf group was significantly higher than 1. The activity of Na + -K + ATPase in 1% Enf group was significantly lower than that in 1.1% Enf group (P <0.05 or 0.01), while the ADP content and EC in 2.2% Enf group were significantly higher than 1.1 % Enf group, Ca2 + ATPase activity was significantly lower than the 1.1% Enf group (P <0.05 or 0.01). Conclusion Enf can inhibit cardiomyocyte electrophysiological activity by inhibiting myocardial ATPase activity, and hypothermia may increase the myocardial inhibitory effect of enflurane.