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目的探讨高果糖诱发的胰岛素抵抗大鼠认知功能、胆碱乙酰转移酶(choline acetyltrans-ferase,ChAT)活性及胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)的变化及吡格列酮的干预作用。方法从45只Wistar大鼠中随机选取10只作为对照组(NC组);其余35只以10%的果糖水诱发胰岛素抵抗;4周后根据胰岛素抵抗指数(insulin resistance index,IRI)将制作成功的胰岛素抵抗模型大鼠随机分为胰岛素抵抗组(IR组)和吡格列酮组(PIO组)。于干预12周末通过Morris水迷宫实验观察大鼠认知功能改变情况;采用化学比色法测定脑组织内ChAT活性;Western blotting检测脑组织IGF-1的表达;对脑组织切片进行尼氏染色。结果PIO组与IR组逃避潜伏期较NC组明显延长(分别为P<0.01,P<0.05),PIO组较IR组明显缩短(P<0.01);PIO组与IR组ChAT活性较NC组降低(P<0.01,P<0.01),PIO组较IR组增强(P<0.05);PIO组与IR组IGF-1表达较NC组降低(P<0.01),PIO组IGF-1表达较IR组增加(P<0.05)。结论吡格列酮对IR模型大鼠的学习记忆功能减退具有改善作用,其机制可能与促进神经元ChAT、IGF-1的表达,维持胆碱能神经功能正常有关。
Objective To investigate the changes of cognitive function, activity of choline acetyltrans-ferase (ChAT) and insulin-like growth factor-1 (IGF-1) in rats with insulin resistance induced by high fructose And pioglitazone intervention. Methods Ten randomly selected 45 Wistar rats were used as the control group (NC group). The remaining 35 rats were induced with 10% fructose water for insulin resistance. After 4 weeks, insulin resistance index (IRI) Of insulin resistance model rats were randomly divided into insulin resistance group (IR group) and pioglitazone group (PIO group). The changes of cognitive function in rats were observed by Morris water maze test at 12 weeks after intervention. The activity of ChAT in brain tissue was measured by chemical colorimetry. The expression of IGF-1 in brain tissue was detected by Western blotting. The brain tissue sections were subjected to Nissl staining. Results The escape latency of PIO group and IR group was significantly longer than that of NC group (P <0.01, P <0.05, PIO group, PIO group and IR group were significantly shorter than that of NC group (P <0.01, P <0.01). The expression of IGF-1 in PIO group was significantly lower than that in NC group (P <0.01) (P <0.05). Conclusion Pioglitazone can ameliorate the learning and memory deficits in IR rats. The mechanism may be related to the promotion of the expression of ChAT and IGF-1 and the maintenance of cholinergic function.