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对高中生物的几个实验,已有不少有效的改进方法,为更好地获得实验效果,我也谈些在实验过程中得到的改进体会,以供参考。一、洋葱根尖细胞的有丝分裂装片观察1.剪取洋葱根尖约3—5毫米,用刀片细心地把它纵剖成数片,这样便于把根尖切片压成单层的细胞,以提高观察效果。2.用镊子把剖成薄片的根尖放于载玻片的中央,用10％的盐酸解离,解离后用吸水纸把盐酸吸去,再滴以清水漂洗,然后用吸水纸吸去,重复数次,时间约十分钟,再滴以1％龙胆紫溶液染色3分钟,用吸水纸吸去。这样,解离、漂洗、染色不用镊子夹取,可避免夹破,
For several experiments on high school biology, there have been many effective methods of improvement. In order to obtain better experimental results, I also talked about some improvements in the experimental process for reference. 1. Observation of the mitotic loading of the root tip cells of onion 1. Cut the root tip of the onion about 3-5 mm, carefully slice it into several pieces with a blade, so that it is convenient to press the apical slice into a single layer of cells. Improve observation. 2. Use tweezers to place the tip of the sliced piece in the center of the slide and dissociate with 10% hydrochloric acid. After dissociation, remove hydrochloric acid with absorbent paper, rinse with clean water, and blot with absorbent paper. Repeat several times for about 10 minutes. Drip again with 1% gentian violet solution for 3 minutes and blot with absorbent paper. In this way, dissociation, rinsing, and dyeing can be avoided without tweezers.