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目的:探讨睡眠剥夺(SD)对大鼠凝血功能及血红蛋白的影响.方法:将32只健康成年雄性SD大鼠(220±10)g采用随机数字表法分为正常笼饲养组(CN组)、大平台饲养组(TC组)、睡眠剥夺组(SD组)及睡眠剥夺恢复组(SR组),每组各8只.采用改良多平台睡眠剥夺法(MMPM)建立睡眠剥夺模型,连续剥夺5 d,颈内静脉采血,置于抗凝管内,采用全自动血液分析仪进行血细胞计数,包括白细胞总数(WBC)、红细胞总数(RBC)、血红蛋白(Hb)含量及血小板(PLT)计数;采用自动分析仪检测止血参数,包括活化的部分凝血活酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)及纤维蛋白原(FIB).结果:与CN组和TC组比较,SD组大鼠毛色暗淡,易激惹,体重减轻(P＜0.05);WBC明显增加(P＜0.05),RBC及Hb含量明显减少(P＜0.05),PLT差异无统计学意义(P＞0.05);APTT、PT及TT值明显延长(P＜0.05),FIB值明显降低(P＜0.05).与SD组比较,SR组大鼠WBC明显下降(P＜0.05),RBC及Hb含量明显增加(P＜0.05),PLT及体重差异无统计学意义(P＞0.05); APTT、PT及TT值明显缩短(P＜0.05), FIB值明显升高(P＜0.05).SR组与CN组及TC组比较,体重明显减轻(P＜0.05),其余值差异无统计学意义(P＞0.05).CN组与TC组比较,体重、WBC、RBC、Hb、 PLT、APTT、PT、TT及FIB值均差异无统计学意义(均P＞0.05).结论:持续长时间SD可能导致大鼠凝血功能障碍及贫血倾向,睡眠剥夺后可通过延长合适的睡眠时间来改善机体的凝血功能障碍及贫血倾向.“,”Objective : To investigate the effects of sleep deprivation on coagulation function and hemoglobin in rats. Method:Pathogen-free male Sprague-Dawley rats,weighing (220±10)g,were used in this study.32 rats were randomly divided into four groups(n = 8) using a random number table: normal group (group CN), large platform group(group TC),sleep deprivation group(group SD)and sleep recovery group (group SR). The sleep deprivation model was established by a modified multi-platform method(MMPM). Five days after continuous deprivation, the jugular vein was collected and placed in the anticoagulant tube. The blood cell count, including white blood cell count( WBC),red blood cell count (RBC),hemoglobin ( Hb) and platelet (PLT) were measured by automatic blood analyzer. The coagulation was measured by automatic blood analyze, including activated partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT)and fiber proteins(FIB). Result: Compared with group CN and group TC,group SD had dark coat,irritable and weight loss(P＜0.05);white blood cell count(WBC)increased significantly(P＜0.05), red blood cell count (RBC) and hemoglobin( Hb) decreased significantly (P＜0.05), PLT had no significant change; APTT,PT and TT values were significantly prolonged(P＜0.05),FIB value was significantly lower(P＜0.05). Compared with group SD,body weight of rats in group SR had no significant change(P＞ 0.05), WBC decreased significantly(P＜0.05), RBC and Hb content increased significantly(P＜0.05), and PLT had no significant change(P＞0.05); APTT,PT and TT values were significantly shorter(P＜0.05), FIB values were significantly higher(P＜0.05). Compared with group CN and group TC,the body weight of group SR was significantly reduced(P＜0.05),and the rest values did not change significantly. There were no significant differences in body weight,WBC,RBC,Hb,PLT,APTT,PT,TT and FIB between group CN and group TC(P＞0.05). Conclusion : Sleep deprivation may lead to coagulopathy and anemia. After sleep deprivation, coagulation dysfunctionand and anemia tendency can be improved by prolonging appropriate sleep time.