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选用定位于普通小麦7个部分同源群上的276对微卫星引物对普通小麦中国春和簇毛麦的基因组DNA进行扩增分析,有148对引物可在两个物种间检测到多态性。利用上述显示多态性的引物进一步对7个中国春-簇毛麦二体附加系进行扩增分析,筛选出分别可用来追踪簇毛麦1V至7V染色体的引物wmc49(1BS)、wmc25(2BS)、gdm36(3DS)、gdm145(4AL)、 wmc233(5DS)、wmc256(6AL)和gwm344(7BL)。此外还发现6DS上的微卫星引物gwm469可以用来追踪簇毛麦的2V染色体;2DS上的微卫星引物gdm107可用来追踪簇毛麦的6V染色体。进一步用涉及不同簇毛麦和小麦背景的小麦-簇毛麦染色体附加系、代换系和易位系进行扩增分析,这些微卫星标记也可用来鉴定簇毛麦的各条染色体。因此,这些簇毛麦染色体特异的微卫星标记可用来追踪普通小麦背景中的簇毛麦染色体。
A total of 276 pairs of microsatellite primers were used to amplify the genomic DNA of common Chinese spring and cluster hay, which were located on seven partial homoeologous groups of common wheat. There were 148 pairs of primers that could detect polymorphism between the two species . The above-mentioned polymorphic primers were used to further amplify the additional lines of seven Chinese Spring-Hamster Meriones. The primers wmc49 (1BS) and wmc25 (2BS ), Gdm36 (3DS), gdm145 (4AL), wmc233 (5DS), wmc256 (6AL) and gwm344 (7BL). In addition, it was found that the microsatellite primer gwm469 on 6DS can be used to track the chromosome 2V of cluster hay; the microsatellite primer gdm107 on chromosome 2DS can be used to trace the 6V chromosome of cluster hay. The microsatellite markers were also used to identify the chromosomes of H. rhamnosus. The microsatellite markers were also used to amplify the wheat-cluster-mahogany adventitious lines, substitution lines and translocation lines. Therefore, these cluster-wool chromosome-specific microsatellite markers can be used to track cluster-wool chromosomes in common wheat backgrounds.