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目的探讨大气PM_(2.5)引起的小鼠心肺组织的氧化损伤。方法将24只健康6周龄SPF级C57BL/6雄性小鼠按体重随机分为4组,分别为空白对照(无菌生理盐水)组和1.5、7.5、37.5 mg/kg PM_(2.5)染毒组,每组6只。染毒采用一次性气管注入法,染毒容量为1.5 ml/kg。染毒24 h后,检测小鼠心、肺组织超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力以及谷胱甘肽(GSH)和丙二醛(MDA)含量。同时,分析PM_(2.5)主要化学组分。结果所采集的PM_(2.5)样品主要化学组分浓度占比分别为有机物(24.8%)、元素碳(4.94%)、SO_4~(2-)(12.2%)、NO_3~-(15.3%)、NH_4~+(2.39%)、矿物尘(8.09%)、金属元素(1.61%)。与对照组比较,37.5 mg/kg PM_(2.5)染毒组小鼠心组织MDA含量较高,且CAT、SOD活力较低;而7.5、37.5 mg/kg PM_(2.5)染毒组小鼠心组织GSH含量均较低,差异均有统计学意义(P<0.05)。与对照组比较,7.5、37.5 mg/kg PM_(2.5)染毒组小鼠肺组织MDA含量均较高,差异均有统计学意义(P<0.05);而各剂量PM_(2.5)染毒组小鼠肺组织GSH的含量及CAT、SOD的活力均无明显改变。结论高浓度PM_(2.5)急性暴露可诱发小鼠心、肺组织的氧化应激反应。
Objective To investigate the oxidative damage of heart and lung tissue induced by PM 2.5 in mice. Methods Twenty-four healthy 6-week-old C57BL / 6 male SPF mice were randomly divided into 4 groups according to body weight: blank control (sterile saline) and 1.5, 7.5, 37.5 mg / kg PM 2.5 Group, 6 in each group. The use of a one-time injection of tracheal injection, exposure capacity of 1.5 ml / kg. Twenty-four hours after exposure, the activities of superoxide dismutase (SOD) and catalase (CAT) and the content of glutathione (GSH) and malondialdehyde (MDA) in heart and lung tissue of mice were measured. At the same time, the main chemical components of PM_ (2.5) were analyzed. Results The concentrations of major chemical components in PM 2.5 samples collected were 24.8%, 4.94%, 12.2% SO 3 2-, 15.3% SO 3 2-, NH4 ~ + (2.39%), mineral dust (8.09%), metal elements (1.61%). Compared with the control group, the content of MDA in the heart of the mice exposed to 37.5 mg / kg PM 2.5 was higher than that of the control group, and the activity of CAT and SOD was lower. In the 7.5, 37.5 mg / kg PM 2.5 group, The content of GSH in the tissue was lower, the differences were statistically significant (P <0.05). Compared with the control group, the content of MDA in lung tissue of mice exposed to 7.5,37.5 mg / kg PM 2.5 was higher than that of the control group (P <0.05), while the PM 2.5 group The content of GSH and the activity of CAT and SOD in lung tissue of mice had no obvious changes. Conclusion The acute exposure to high concentration PM 2.5 can induce oxidative stress in the heart and lung of mice.