论文部分内容阅读
目的:建立高效液相色谱法亚贡叶中亚贡二萜酸A的含量。方法:采用Agilent ZORBAX SB-C18(4.6 mm×150 mm,5μm)色谱柱,以乙腈-0.5%磷酸水溶液(35∶65)为流动相,流速1.0 m L·min-1,检测波长203 nm,柱温30℃。结果:亚贡二萜酸A的线性范围为0.064~1.028μg,r=0.999 9;加样回收率为99.6%,RSD为1.5%;批号为20101001、20101015、20101020的3批样品中亚贡二萜酸A含量分别为0.531、0.654、0.294 mg·g-1。结论:不同来源的亚贡叶的质量差异较大;方法学验证结果表明本法适用于亚贡叶的质量控制。
OBJECTIVE: To establish a HPLC method for the determination of diketonic acid A in Gong Ya Ya Gong Ye. METHODS: The mobile phase was acetonitrile (0.5% phosphoric acid) (35:65) at a flow rate of 1.0 mL · min-1 using an Agilent ZORBAX SB-C18 column (4.6 mm × 150 mm, 5 μm) Column temperature 30 ℃. Results: The linear range of diterpene acid A was 0.064 ~ 1.028μg, r = 0.999 9; the recovery rate was 99.6% and the RSD was 1.5%. Three batches of samples of 20101001, 20101015 and 20101020 The contents of terpene A were 0.531,0.654,0.294 mg · g-1, respectively. Conclusion: The quality of Ya Gong leaves from different sources is quite different. The verification results show that this method is suitable for the quality control of Ya Gong leaves.