论文部分内容阅读
目的研究慢性应激状态对老年性骨质疏松症病变程度的影响,为老年性骨质疏松症的预防和治疗提供可靠的实验依据。方法选取SPS级快速老化小鼠SAM-P6(3月龄,雄性)24只,随机分为实验组和对照组(n=12),实验组每日给予不可预见性慢性温和应激,对照组正常饲养,4周后检测血清学指标和micro-CT各项参数,综合评估骨代谢水平和骨强度。结果实验4周末,实验组小鼠体重比实验开始时明显下降,差异有统计学意义(P<0.05);与对照组比较,实验组血钙、碱性磷酸酶水平均显著增加(P<0.05),血清骨钙蛋白水平明显降低(P<0.05),而血清Ⅰ型胶原氨基末端肽水平显著增加(P<0.05),骨密度明显降低(P<0.05),骨小梁相对体积显著降低(P<0.01),骨小梁数目明显减少(P<0.05)。结论慢性应激状态加剧骨丢失,降低骨强度,加重老年性骨质疏松症严重程度,在老年性骨质疏松症的预防和治疗过程中应予以重视。
Objective To study the effect of chronic stress on the degree of senile osteoporosis and to provide a reliable experimental evidence for the prevention and treatment of senile osteoporosis. Methods Twenty-four SAM-P6 (3 months old, male) mice were randomly divided into experimental group and control group (n = 12). The rats in experimental group were given unforeseen chronic mild stress and control group Normal feeding, 4 weeks after the detection of serological indicators and micro-CT parameters, a comprehensive assessment of bone metabolism and bone strength. Results At the end of the experiment 4 weeks, the body weight of experimental mice decreased significantly compared with that at the beginning of the experiment, the difference was statistically significant (P <0.05); Compared with the control group, the levels of serum calcium and alkaline phosphatase were significantly increased (P <0.05 (P <0.05). The serum level of amino-terminal peptide of type Ⅰ collagen significantly increased (P <0.05), bone mineral density decreased significantly (P <0.05), and the relative volume of trabecular bone decreased significantly P <0.01), the number of trabecular significantly decreased (P <0.05). Conclusion Chronic stress exacerbates bone loss, decreases bone strength and aggravates the severity of senile osteoporosis. It should be emphasized in the prevention and treatment of senile osteoporosis.