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目的 研究快速上浮脱险致减压病动物凝血纤溶系统的变化,从而为快速上浮脱险减压病筛选能反映其损伤程度的敏感指标.方法 雄性SD大鼠80只,按数字表法随机分为2组,正常对照组(对照组)20只,快速上浮脱险实验组(实验组)60只,根据动物是否死亡进而分为死亡组和存活组.实验组以21/7指数速率加压至1.5 MPa(表压),停留4 min后匀速减压至常压.观察实验组大鼠出舱后30 min内的存活状况.如果大鼠30 min内死亡,则立即抽取其下腔静脉血;如果30 min内未死亡,则在30 min后抽取其下腔静脉血,血标本置于3.5％枸橼酸钾及1％肝素内抗凝,用于检测凝血6项指标和血小板计数.结果 实验组大鼠30 min内死亡率30只(50％).对照组、存活组与死亡组大鼠的血小板计数比较差异无统计学意义(P＞0.05);存活组、死亡组及对照组大鼠的凝血酶时间分别为(28.00±1.69)s、(27.21 ±3.82)s与(30.91±2.82)s,组间比较差异均有统计学意义(P＜0.01),对照组大鼠的纤维蛋白原(2.91±0.43)g/L和死亡组(3.72±1.55)g/L比较差异有统计学意义(P＜0.05),存活组大鼠的D-二聚体(0.19±0.05) μg/L和死亡组(0.24±0.08) μg/L比较差异有统计学意义(P＜0.05).结论 凝血酶时间、纤维蛋白原和D-二聚体可综合反映快速上浮脱险致减压病的发生以及造成损伤的严重程度,可作为反映损伤程度的指标.“,”Objective To study changes in coagulation and fibrinolysis system of animals with decompression sickness caused by fast buoyancy ascent escape.Methods Eighty male SD rats were randomly divided into 2 groups:the normal control group (n =20),the fast buoyancy ascent escape group (or simply the experimental group) (n =60) and the experimental group was further divided into the death and survival groups,based on whether the animals died or survived after escape.The animals in the experimental group were compressed to a pressure of 1.5 MPa at an exponential rate of 2t/7 with compressed air,then,after staying for 4 min at thc above pressure,the animals were decompressed to atmospheric pressure at linear speed.The animals that survived within 30 min was observed after they were brought out of the chamber.If the animals died within 30 min,blood samples were immediately collected from inferior vena cava.If death did not occur within 30 min,then blood samples were collected 30 min later.The collected blood samples were placed in 3.5％ potassium citrate or 1％ heparin for anticoagulation,for the detection of coagulation and platelet count.Results The mortality for the animals in the experimental group was 50％.No statistical significance could be seen in platelet counts,when comparisons were made between the 3 groups,but there was statistical significance in coagulation time,when comparisons were made between the 3 groups.There were statistical differences in the lcvels of fibrinogen,when comparisons were made between the normal control group (2.91 ± 0.43) g/L and the death group (3.72 ± 1.55)g/L.And statistical significance could also be noted in the levels of D-dimer,when comparisons were made between the survival group (0.19 ± 0.05) μg/L and the death group (0.24 ± 0.08) μg/L.Conclusions Coagulation time,fibrinogen and D-dimer could be used as indicators for the occurrence of DCS induced by fast buoyancy ascent escape,and seriousness of damage as well.