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近代柱层析填料的改进,使经典的液相色谱发展成高压液层,同时吸附剂的改进也导致了高效薄层层析(High Performance Thin-Layer Chromatography 简称 HPTLC)的出现。所谓 HPTLC 实际上是吸附剂粒子的粒度细而均匀,一般直径平均为5μ的一种薄层。薄层展开以后,所显现的斑点小而圆整。对混合组分的分离效能好,移动距离只需3~7cm,十几分钟就能完成一次层析。灵敏度较高,分
The improvement of the modern column chromatographic packing has led to the development of classical liquid chromatography as a high-pressure liquid layer. At the same time, the improvement of adsorbent has also led to the appearance of High Performance Thin-Layer Chromatography (HPTLC). The so-called HPTLC is actually a thin and uniform particle size of the adsorbent particles, generally a thin layer with an average diameter of 5μ. After the thin layer is unrolled, the spots appearing are small and round. The separation performance of the mixed components is good, the moving distance is only 3 ~ 7cm, and a chromatography can be completed in ten minutes. High sensitivity, points