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干旱应答元件结合蛋白(dehydration responsive element binding protein,DREB),在植物应对干旱、盐碱和低温胁迫的反应中起非常重要的调控作用。利用已知的甘蔗栽培种DREB2转录因子序列,设计引物,按照同源克隆的方法,获得了2个割手密DREB2基因组DNA序列,分别命名为Ss DREB2-a和Ss DREB2-f(Gen Bank登录号分别为:KU963272和KU963277)。序列分析结果表明,Ss DREB2-a基因序列全长为1 578 bp,Ss DREB2-f基因序列全长为1 729 bp,2个基因均包含1个内含子和2个外显子。Ss DREB2-a和Ss DREB2-f基因的c DNA序列全长分别为824 bp和971 bp,均编码262个氨基酸。序列比对分析显示,2个基因的序列相似性为96.6%,编码的蛋白相似性为98.9%,存在3个氨基酸变异位点。系统进化分析结果显示,割手密DREB2转录因子与高粱、牛鞭草、斑茅、玉米等植物的DREB2转录因子的同源关系最近。基因的获得为下一步了解DREB2基因表达与割手密抵御非生物胁迫能力之间的关系奠定了基础。
DREB plays a very important role in plant response to drought, salinity and low temperature stress. Using known DREB2 transcription factor sequences of sugarcane cultivars, primers were designed and two DREB2 genomic DNA sequences were obtained by homologous cloning and named as Ss DREB2-a and Ss DREB2-f No. were: KU963272 and KU963277). Sequence analysis showed that the full length of Ss DREB2-a gene was 1 578 bp and the full length of Ss DREB2-f gene was 1 729 bp. Both of the two genes contained one intron and two exons. The full-length c DNA sequences of Ss DREB2-a and Ss DREB2-f genes were 824 bp and 971 bp, both encoding 262 amino acids. Sequence alignment analysis showed that the sequence similarity of the two genes was 96.6%, the similarity of the encoded proteins was 98.9%, and there were three amino acid variation sites. Phylogenetic analysis showed that the homology of DREB2 transcription factor with the DREB2 transcription factor in plants such as sorghum, verbena, dactylis, maize and so on was the most recent. Gene access for the next step to understand the DREB2 gene expression and cut the secret density to resist the relationship between abiotic stress laid the foundation.