Brassica rapa cultivars Qinghaidahuang and Menyuan small rape were used as female parents and separately interspecifically hybridized with Zhongchijielan to artificially synthesize a number of Brassica napus resources.Observation of meiosis,pollen fertility and pollen tube germination and analysis of self-compatibility index of these artificial B.napus varieties were conducted.Results showed that pollen mother cells of artificial B.napus lines from two cross combinations all had normal chromosome behavior and high pollen fertility,but the selfing seed-setting and self-compatibility index varied extremely significantly;B.napus lines from cross combination Qinghaidahuang×Zhongchijielan are highly self-compatible,while B.napus lines from cross combination Menyuan small rape×Zhongchijielan are highly self-incompatible.This study analyzed the heredity laws of self-incompatibility trait in artificially synthesized lines and discussed application prospects of artificially synthesized self-incompatible B.napus lines in rapeseed production. Brassica rapa cultivars Qinghaidahuang and Menyuan small rape were used as female parents and separately interspecifically hybridized with Zhongchijielan to artificially synthesize a number of Brassica napus resources. Observation of meiosis, pollen fertility and pollen tube germination and analysis of self-compatibility index of these artificial B .napus varieties were conducted. Results showed that pollen mother cells of artificial B. napus lines from two cross combinations all had normal chromosome behavior and high pollen fertility, but the selfing seed-setting and self-compatibility index varied significantly significantly; B. napus lines from cross combination Qinghaidahuang × Zhongchijielan are highly self-compatible, while B. napus lines from cross combination Menyuan small rape × Zhongchijielan are highly self-incompatible. This analysis analyzed the heredity laws of self-incompatibility trait in artificially synthesized lines and discussed application prospects of artificially synthesized self-i ncompatible B.napus lines in rapeseed production.