论文部分内容阅读
本文用Ames试验TA_(98)、TA_(100)两菌株,对大量存在于环境中的腐殖酸的抑制突变效应进行了研究。经超滤将腐殖酸按分子量的大小分为四个部分:<1000、1000～10000、10000～300000、>300000。每部分均用蒸馏水配成浓度为 4000 ppm的检液。其中,>300000的部分因含有颗粒而为悬浮液,取适量该悬浮液经离心而得上清液。然后经高压灭菌做Ames试验。抗突变试验:将隔夜孵育的菌液5 ml,用pH为7.0,1/15 M的冷磷酸盐缓冲液(PB)离心冲洗两次,后取出该菌液3 ml,加入200μg/0.2 ml的B[a]P、3 ml S_937℃,慢振荡孵
In this paper, Ames test TA_ (98), TA_ (100) two strains of humic acid in a large number of existing in the environment of the inhibitory mutation effect were studied. Humus by ultrafiltration according to the size of the molecular weight is divided into four parts: <1000,1000 ~ 10000,10000 ~ 300,000,> 300,000. Each part is dosed with distilled water to a concentration of 4000 ppm of the test solution. Among them,> 300000 part because of containing particles as a suspension, the right amount of the suspension obtained by centrifugation supernatant. Then Ames test was done by autoclaving. Anti-mutation test: 5 ml of bacterial liquid incubated overnight was centrifuged twice with cold phosphate buffer (PB) at pH 7.0 and 1/15 M, 3 ml of the bacterial liquid was removed, and 200 μg / 0.2 ml B [a] P, 3 ml S_937 ° C, slow shaking incubation