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目的:研究Ⅰ类整合子在肺炎克雷伯菌分布、结构及其在介导耐药中的作用。方法:利用多重聚合酶链反应(PCR)方法检测整合酶基因,对其阳性菌株可变区扩增产物进行测序分析;采用微量稀释法测定14种抗生素对试验菌株的敏感性。结果:在其中38株肺炎克雷伯菌DNA上检测到I类整合,阳性率43.2%。所携带的耐药基因盒绝大多数为aadA2和dfr17,整合子阳性组耐药率明显高于阴性组(P<0.05)。结论:目前临床分离的肺炎克雷伯菌耐药性强,并广泛存在I类整合子。菌株携带基因盒和耐药表型之间有较好的对应关系,基因盒介导了细菌耐药性。耐药基因盒是整合子阳性菌株对氨基糖苷类耐药的主要原因。
Objective: To study the distribution and structure of class Ⅰ integron in Klebsiella pneumoniae and its role in mediating drug resistance. Methods: The integrase gene was detected by multiplex polymerase chain reaction (PCR), and the amplified product of the variable region of the positive strain was sequenced. The sensitivity of 14 antibiotics to the tested strains was determined by microdilution method. Results: Type I integrons were detected in 38 strains of Klebsiella pneumoniae with a positive rate of 43.2%. Most of the resistant gene cassettes carried were aadA2 and dfr17, and the positive rate of integron positive group was significantly higher than that of negative group (P <0.05). CONCLUSION: Klebsiella pneumoniae is currently clinically isolated with high resistance and widespread class I integron. The strain carries a good correspondence between the gene cassette and the resistant phenotype, and the gene cassette mediates bacterial resistance. Drug-resistant gene cassettes are the major contributors to the resistance of aminoglycosides to integron-positive strains.