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以花溪辣椒、遵义辣椒为试材,辣椒白粉病菌为供试菌,苯丙噻二唑(BTH)为诱导剂,采用喷施接种法,研究了BTH诱导辣椒对白粉病产生抗性的作用机制。结果表明:不同浓度BTH均可以诱导辣椒对白粉病产生抗性,浓度为0.20mmol·L-1时诱导效果最佳,防治效果为81.82%;BTH-接菌处理的辣椒叶片过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性明显增加;BTH-接菌、BTH-未接菌和CK-接菌处理都能诱导辣椒叶片几丁质酶和β?1,3-葡聚糖酶活性的升高,但CK-接菌处理增加幅度较小。BTH不影响白粉病菌孢子萌发和附着胞子的产生,却能有效抑制菌丝的生长,表现为菌丝生长缓慢和菌丝的侧枝数减少。通过扫描电镜观察,发现CK-接菌处理在24h后,辣椒叶片保护层开始被溶解,BTH-接菌处理的辣椒在接菌48h后才出现该现象,说明BTH能产生阻碍或延迟菌丝破坏的保护层物质。
Taking Huaxi pepper and Zunyi pepper as test materials, pepper powdery mildew as tested bacteria and benzothiadiazole (BTH) as inducer, the mechanism of BTH-induced pepper resistance to powdery mildew was studied by spraying inoculation method . The results showed that BTH could induce the resistance of pepper to powdery mildew. The optimal concentration of 0.20mmol·L-1 was 81.82%, the effect of BTH-inoculated pepper leaves peroxidase POD, PPO and PAL were significantly increased. BTH-inoculation, BTH-inoculation and CK-inoculation could induce chitinase And β-1, 3-glucanase activity increased, but CK-inoculation increased less. BTH did not affect spore germination and attachment of spores of powdery mildew, but effectively inhibited the growth of mycelia, which showed slow growth of mycelia and decreased number of collateral of hypha. Scanning electron microscopy showed that the protective layer of pepper leaves began to be dissolved after treatment with CK-inoculation for 24 hours. This phenomenon was observed only after 48 hours of inoculation with BTH-inoculated bacteria, indicating that BTH could hinder or delay mycelium damage Protective layer material.