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试验从温度、pH值、光照、碳氮源的利用等方面对玉米圆斑病病原菌的生物学特性进行了较为系统的研究.结果表明:玉米圆斑病菌菌丝在5~40℃之间均能生长,而在10~35℃之间能产孢,最适生长和产孢温度为25℃,菌落直径达62 mm,产孢量为1.04×105(个/mL);病原菌在pH 2~12之间均能生长和产孢,其中在pH为7时病原菌菌丝生长最好,菌落直径达35.3 mm,pH为8时病原菌产孢量最大,为2.06×105(个/mL);光暗交替有利于病原菌产孢,光照对菌丝的扩展基本没影响;该病原菌对单糖、双糖、多糖等碳源以及有机氮和无机氮均能利用,其中以乳糖作碳源、甘氨酸作氮源时菌丝生长最佳,以D-木糖作碳源、以牛肉膏作氮源时产孢最好.
The biological characteristics of pathogen of Circular Dwarf corniculatum were systematically studied in terms of temperature, pH value, light and the utilization of carbon and nitrogen sources.The results showed that the mycelia of Cryphrouridae were between 5 and 40 ℃ The growth and sporulation temperature ranged from 10 ℃ to 35 ℃. The optimal temperature for growth and sporulation was 25 ℃, the diameter of the colony was 62 mm and the sporulation volume was 1.04 × 105 (mL / mL) 12 were able to grow and produce spores. Among them, mycelial growth was the best at pH 7 with 35.3 mm in diameter and 2.06 × 105 spores / mL at pH 8 Dark alternation is conducive to the sporulation of pathogenic bacteria, light basically does not affect the expansion of mycelium; the pathogen on monosaccharides, disaccharides, polysaccharides and other carbon sources as well as organic nitrogen and inorganic nitrogen can use, of which lactose as carbon source, glycine Nitrogen source mycelium growth best, with D-xylose as a carbon source, beef extract as a nitrogen source spores the best.