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用反相HPLC同时测定红葡萄酒中白藜芦醇及糖苷4种异构体。以 OASISTM固相萃取柱进行前处理,以 Hypersil C18(4.6 mm×250mm,5μm)为色谱柱,乙睛-水(含 0.1%三乙胺)梯度洗脱,同时用二极管阵列检测器在288nm和 308nm检测。结果表明:白藜芦醇及其糖苷4种异构体在 20 min内可得到较好的分离,其回收率均在(95.7±1.4)%以上,最小检测限为0.001 mg·L-1。
Simultaneous determination of four isomers of resveratrol and glycoside in red wine by reverse phase. The column was pre-treated with OASISTM SPE cartridge. The column was eluted with a Hypersil C18 column (4.6 mm × 250 mm, 5 μm) and a gradient of acetonitrile-water (containing 0.1% triethylamine) 308nm detection. The results showed that four isomers of resveratrol and its glycoside could be separated within 20 min. The recoveries of resveratrol and its glycosides were above 95.7 ± 1.4% with the minimum detectable limit of 0.001 mg · L -1.