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目的 探讨Ⅱ型树突状细胞 (typeII dendriticcells,DC2 )数量和功能的变化及其与乙型肝炎 (乙肝 )患者病程、淋巴细胞亚群和HBVDNA病毒载量的关系。方法 采用流式细胞分析技术对 10 3例乙肝患者 (包括HBV携带者 11例 ,慢性乙肝轻度 35例 ,中度 33例 ,重度 13例 ,急性乙肝11例 )和 2 5例健康人 (对照组 )的外周血DC2进行检测 ,同时检测患者血常规 ,计算出DC2绝对数 ;用体外灭活的 1型单纯疱疹病毒 (HSV 1)刺激外周血单个核细胞 (PBMC)并与PBMC进行 2 4h共培养 ,检测上清中DC2的α干扰素产生能力 ;统计分析DC2数量、功能变化及其临床意义。结果 对照组外周血DC2占PBMC比例为 0 32 %± 0 13% ,绝对数为 (7 2± 2 4 ) 10 6个 /L。与对照组比较 ,HBV携带者DC2的比例 (0 32 %± 0 12 % )和绝对数 (6 9± 3 4 ) 10 6个 /L无明显下降 ;急性乙肝患者的DC2比例和绝对数下降 ,但无统计学意义 ;而慢性肝炎患者DC2的比例和绝对数随病程加重而降低 ,中、重度肝炎的DC2下降较轻度更为显著。DC2产生α干扰素的功能在急、慢性乙肝患者及HBV携带者均降低 ,各组患者之间差异不显著 ;DC2的数量与HBVDNA病毒载量未发现相关性 ,但与自然杀伤 (NK)细胞及CD8+ T数量高低存在正相关。结论 慢性乙肝患者外周血DC2数量和?
Objective To investigate the changes in the number and function of type II dendritic cells (DC2) and their relationship with the course of disease, lymphocyte subsets and HBVDNA in patients with hepatitis B (hepatitis B). Methods Flow cytometric analysis was performed on 103 patients with HBV (11 carriers including HBV carriers, 35 mild chronic hepatitis B patients, 33 moderate patients, 13 severe patients and 11 acute hepatitis B patients) and 25 healthy controls (DC2) in peripheral blood of the patients were detected, and the blood routine of the patients was detected to calculate the absolute number of DC2. Peripheral blood mononuclear cells (PBMCs) were stimulated with in vitro-inactivated herpes simplex virus type 1 (HSV 1) and incubated with PBMC for 24 h Co-cultured to detect DC2 in the supernatant of interferon-producing capacity; statistical analysis of DC2 quantity and function changes and its clinical significance. Results The ratio of DC2 to PBMC in the control group was 0 32% ± 0 13% and the absolute number was (7 2 ± 2 4) 10 6 cells / L. Compared with the control group, the proportion of DC2 in HBV carriers (0 32% ± 0 12%) and the absolute number (6 9 ± 3 4) 10 6 cells / L did not significantly decrease; the proportion and absolute number of DC2 in patients with acute hepatitis B decreased, But no statistical significance; while the proportion and absolute number of DC2 in patients with chronic hepatitis decreased with the increase of the course of disease, moderate and severe hepatitis DC2 decreased more mildly more significant. DC2 produced interferon alpha in patients with acute and chronic hepatitis B and HBV carriers were reduced, the difference was not significant between the groups of patients; the number of DC2 and HBVDNA viral load was not found, but with natural killer (NK) cells And CD8 + T level there is a positive correlation. Conclusion Chronic hepatitis B patients with peripheral blood DC2 quantity and?