培养基内含有10％二甲基亚砜,是通常用於低温保存细胞的保存液,但对利什曼原虫前鞭毛体的毒性较大,复苏后仅有10％左右的前鞭毛体存活。本文介绍一种简便无毒的保存液,即在含有20％小牛血清的199培养液内加入10％甘油,经过滤消毒备用。利什曼原虫前鞭毛体经pH7.4PBS洗涤后,制成1×10~8ml悬液,于10％甘油199保存液中保存,其比例为1:1。分装于塑料管中,每管1ml,置入特制的泡沫盒内,放入 Medium containing 10% dimethylsulfoxide, which is commonly used as a preservative for cryopreserved cells, is more virulent to Leishmania promastigotes and only about 10% of promastigotes survive the recovery. This article describes a simple non-toxic preservation solution, that is, with 20% fetal bovine serum containing 199% 10% glycerol, sterile filtration by filtration. After the Leishmania promastigotes was washed with pH 7.4 PBS, 1 × 10 -8 ml suspensions were prepared and stored in a 10% glycerol 199 stock solution at a ratio of 1: 1. Sub-packed in plastic tubes, each tube 1ml, into a special foam box, into