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为定位大豆蛋白质含量稳定性QTL,从而为培育高蛋白大豆品种提供依据,本研究利用源自美国大豆Charleston和中国品种东农594杂交获得的147个株系组成的重组自交系群体,利用三种生态环境下三年数据估算的Shukla稳定性方差对大豆蛋白含量进行了遗传和QTL分析。结果表明,利用复合区间作图法(CIM)检测大豆蛋白稳定性QTL得到2个QTL,分别为qPRO1-1和qPRO17-1,位于连锁群A1和L上,贡献率分别为4.70%和5.73%,共解释10.43%的表型变异。利用混合区间作图法(MIM)检测到2对上位性QTL,互作染色体为A1×G和A1×A2,上位效应分别为0.19**和-0.22,贡献率为12.82%和17.42%,共解释30.24%表型变异。本实验分析多个环境下的数据,考虑到了QTL与环境的互作效应,在三种环境条件下分析QTL,检测到了在不同环境下可以稳定出现的QTL位点。控制大豆蛋白含量的QTL位点,都表现出明显的上位性效应和GE互作效应。其中稳定性较好的QTL和公共图谱上定位的调控大豆蛋白质含量的QTL prot 1-7、cq oil003、oil8-1、prot 17-5、prot 2-1及prot12-1等在区间上一致。
In order to locate the QTL of soybean protein content stability, and to provide a basis for cultivating high protein soybean varieties, we used 147 inbred lines of inbred lines derived from Charleston, a soybean cultivar in the United States, and Dongnong 594, Shukla stability variances estimated from three-year data under various ecologic environments were used for genetic and QTL analysis of soybean protein content. The results showed that QTL for the stability of soybean protein detected by composite interval mapping (CIM) showed two QTLs, qPRO1-1 and qPRO17-1, located in linkage groups A1 and L, with contribution rates of 4.70% and 5.73% , A total of 10.43% of the phenotypic variation was explained. Two pairs of epistatic QTLs were detected by hybrid interval mapping (MIM). The interaction chromosomes were A1 × G and A1 × A2. The epistatic effects were 0.19 ** and -0.22, respectively, with a contribution rate of 12.82% and 17.42% Explain 30.24% of phenotypic variation. In this experiment, the data of multiple environments were analyzed. Considering the interaction effect between QTL and environment, QTLs were analyzed under three environmental conditions and the QTL loci that could be stably appeared in different environments were detected. The QTL loci that control the protein content of soybeans all showed significant epistatic effects and GE interaction effects. The QTLs with good stability and the QTLs prot 1-7, cq oil003, oil8-1, prot 17-5, prot 2-1 and so on, which are located on the public map and regulated the protein content of soybean, were consistent in the interval.