论文部分内容阅读
目的 研究同一胃腺癌患者的配对胃腺癌组织与非癌胃组织基因差异 ,并对差异基因片段进行克隆 ,Southern印迹杂交。方法 应用随意扩增多态性DNA指纹图谱分析技术 (arbitrarilyprimerpolymerasechainreaction ,AP PCR) ,即臆断引物的无细胞分子克隆技术 ,亦称臆断引物的聚合酶链反应 ,对 5例配对标本作基因组差异分析。结果 与各自配对的非癌胃组织相比 ,所有胃腺癌组织基因组DNA的AP PCR指纹图谱均存在变异 ,并克隆了其中 1例仅存在于肿瘤组织基因组的差异扩增片段PW 2 .2。Southern印迹杂交发现此PW 2 .2也存在于其他部分胃腺癌标本的随机扩增产物中。结论 AP PCR技术可为差异基因分析和克隆提供一快速而有效的方法。
Objective To study the gene differences between paired gastric adenocarcinoma and non-gastric cancer in patients with the same gastric adenocarcinoma and clone and Southern blot hybridization of the differentially expressed genes. Methods Random amplified polymorphic DNA fingerprinting assay (AP PCR) was used to analyze the genomic differences of 5 matched samples using APCR, an achromatic primer-based cell-free molecular cloning technique, also known as primer-based polymerase chain reaction. Results Compared with the matched non-cancerous gastric tissues, there was a variation in the AP PCR fingerprinting of all the gastric adenocarcinoma tissues and a differential amplification fragment PW 2 .2 was found in only one of the two tissues. Southern blotting revealed that this PW2.2 was also found in random amplified products from other parts of gastric adenocarcinoma. Conclusion AP PCR provides a rapid and efficient method for differential gene analysis and cloning.