论文部分内容阅读
A comparative study of the biochemical and biophysical characteristics of myocardial mitochondria was conducted in 16-weeks-old male stroke-prone spontaneously hypertensive rats (SHRsp) and normotensive Wistar-Kyoto control rats (WKY) (n = 6-12). Cardiac and ventricular weights of SHRsp were significantly higher than those of the WKY group. The average amounts of mitochondria (Mit) and sarcoplasmic reticulum (SR) proteins isolated from the ventricle of SHRsp were 37% and 25% greater, respectively, when compared with those of WKY. Mit cytochrome c oxidase activities (μmol / min / mg) were 4.63 ± 0.71 for SHRsp and 5.55 ± 0.50 for WKY myocardium (x± s), so that the activity of the former was significantly reduced by 17% (P<0.01). Mit Ca2- , Mg2-~ATPase activity (μmol / min / mg) of SHRsp was 24% lower than that of WKY myocardium (P<0.01). The fluorescence polarization of myocardial Mit membrane labelled with DPH (1, 6-diphenyl-1, 3, 5-hexatriene) in hypertensive rats was not different from that in con
A comparative study of the biochemical and biophysical characteristics of myocardial mitochondria was conducted in 16-weeks-old male stroke-prone spontaneously hypertensive rats (SHRsp) and normotensive Wistar-Kyoto control rats (WKY) (n = 6-12). Cardiac and The average amounts of mitochondria (Mit) and sarcoplasmic reticulum (SR) proteins isolated from the ventricle of SHRsp were 37% and 25% greater, respectively, when compared with those of WKY. Mitocytochrome c oxidase activities (μmol / min / mg) were 4.63 ± 0.71 for SHRsp and 5.55 ± 0.50 for WKY myocardium (x ± s), so that the activity of the former was significantly reduced by 17% (P <0.01 ) Mit Ca2-, Mg2- ~ ATPase activity (μmol / min / mg) of SHRsp was 24% lower than that of WKY myocardium (P <0.01). The fluorescence polarization of myocardial Mit membrane labeled with DPH diphenyl-1, 3, 5-hexatriene) in hypertensive rats was not differe nt from that in con