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目的:建立测定人血浆中拉莫三嗪浓度的高效液相色谱法。方法:以Agilent C18反相柱(150 mm×4.6 mm,5μm)为色谱柱,流动相为30 mmol·L~(-1)醋酸铵–乙腈–甲醇(30:15:55);流速:1.0 mL·min~(-1);柱温:30℃;检测波长:310 nm。灵敏度为0.01AUFS。以乙酸乙酯与二氯甲烷(75:25)为提取剂。结果:拉莫三嗪的低、中、高(1.0,4.0,16.0μg·mL~(-1))3种浓度平均相对回收率分别为100.1%,99.78%,98.97%,提取回收率分别为73.66%,75.28%,78.61%;日内、日间偏差RSD均低于15%(n=5);分析方法的检测限0.25μg·mL~(-1);线性范围为0.5~24.0μg·mL~(-1)。线性方程:Y=5.277X+1.06,r=0.9995(n=7)。结论:该测定方法对拉莫三嗪的监测和研究具有较高的灵敏度,同时能够对其进行快速准确的判定。
Objective: To establish a HPLC method for the determination of lamotrigine in human plasma. METHODS: The mobile phase consisted of 30 mmol·L -1 ammonium acetate-acetonitrile-methanol (30:15:55) with an Agilent C18 reversed-phase column (150 mm × 4.6 mm, 5 μm) mL · min ~ (-1); column temperature: 30 ℃; detection wavelength: 310 nm. Sensitivity is 0.01AUFS. Ethyl acetate and dichloromethane (75:25) as extractant. Results: The average recoveries of lamotrigine at concentrations of 1.0, 4.0 and 16.0 μg · mL ~ (-1) were 100.1%, 99.78% and 98.97%, respectively. The recoveries were 73.66%, 75.28% and 78.61%, respectively. The intra-day and inter-day deviations RSD were less than 15% (n = 5). The limits of detection were 0.25μg · mL -1 and the linear range was 0.5-24.0μg · mL ~ (-1). Linear equation: Y = 5.277X + 1.06, r = 0.9995 (n = 7). Conclusion: This assay has high sensitivity to the monitoring and research of lamotrigine, and can be used to make rapid and accurate determination of lamotrigine.