本文用反相HPLC法,以TBA(四丁基氢氧化铵)作离子对试剂,用含13％甲醇的磷酸钾缓冲液进行等比洗脱,同步分离测定了肌酸(Cr)、磷酸肌酸(Crp)、次黄嘌呤(HX)、次黄嘌呤核苷(INO)及AMP、ADP、ATP七种标准品的混合物。结果表明,在210nm波长下,10分钟内上述七种物质均完全分开且基线未漂移,重现性好,是一种简便、快速和可靠的方法。 In this paper, the reversed-phase HPLC method was used for the isocratic separation of tetrabutylammonium hydroxide (TBA) as an ion-pairing reagent in a potassium phosphate buffer containing 13% methanol to simultaneously determine creatine (Cr) and creatine phosphate ( Crp), hypoxanthine (HX), hypoxanthine (INO), and a mixture of AMP, ADP, ATP, and seven standards. The results showed that at the wavelength of 210 nm, the above seven substances were completely separated within 10 minutes and the baseline was not drifted. The reproducibility was good. It was a simple, fast, and reliable method.