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目的 探讨炎症性细胞因子TNF α ,IL 1β和IL 6在沙眼衣原体肺感染中的产生及与机体防御的关系。方法 用沙眼衣原体小鼠肺炎株 (MoPn)通过鼻腔感染小鼠 ,用过氧化物酶连接的鼠抗衣原体脂多糖单抗染色HeLa 2 2 9细胞检测衣原体在肺组织的生长 ;通过测定中性粒细胞髓过氧化物酶 (MPO)检测中性粒细胞在小鼠肺组织中的聚集 ;用RT PCR检测小鼠肺组织炎症性细胞因子mRNA表达。结果 MoPn感染后第 2天 ,肺组织有衣原体生长 ,于感染后第 7天达高峰 ,第 2 1天清除感染的衣原体。感染后第 3天 ,前炎症细胞因子TNF α ,IL 1β和IL 6在小鼠肺组织中的表达明显增高 ,IL 1βmRNA表达于感染第 7天后降低 ,而TNF α和IL 6mRNA的表达至感染后第 14天仍维持较高水平。高水平的TNF α ,IL 1β和IL 6表达出现于中性粒细胞浸润的高峰期。结论 衣原体肺感染诱导前炎症细胞因子TNF α ,IL 1β和IL 6的高表达 ,可能具有中性粒细胞趋化活性 ,并参与衣原体感染的免疫防御
Objective To investigate the production of inflammatory cytokines TNFα, IL-1β and IL-6 in lung infection of Chlamydia trachomatis and its relationship with the body defense. Methods Mice were infected with pneumonia of C. trachomatis mouse pneumonia strain (MoPn) and the growth of Chlamydia pneumoniae was detected by peroxidase-linked mouse anti-Chlamydia pneumoniae lipopolysaccharide monoclonal antibody (McAb) The myeloperoxidase (MPO) was used to detect the aggregation of neutrophils in mouse lung tissue. The mRNA expression of inflammatory cytokines was detected by RT-PCR. Results On the second day after MoPn infection, the chlamydia grew in the lung tissue and reached its peak on the 7th day after infection, and the infected chlamydia was cleared on the 21st day. On the third day after infection, the expressions of TNFα, IL 1β and IL 6, the former inflammatory cytokines, were significantly increased in the lungs of mice, IL 1β mRNA expression decreased after 7 days and the expression of TNFα and IL 6 mRNA The first 14 days still maintain a high level. High levels of TNFα, IL1β, and IL6 expression appear at the peak of neutrophil infiltration. Conclusions Chlamydial pneumonia induces the high expression of inflammatory cytokines TNFα, IL 1β and IL 6 prior to induction of pneumonia, which may have neutrophil chemotactic activity and participate in the immune defense of Chlamydial infection