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目的 酪氨酸蛋白激酶ＪＡＫ１和转录因子ＳＴＡＴ３为参与ＩＬ－６诱导的ＪＡＫ／ＳＴＡＴ信号转导途径的两种主要的信号蛋白分子。本研究试图揭示ＪＡＫ１在ＪＭＫ／ＳＴＡＴ途径诱导活化中的作用。方法 分别采用凝胶阻滞电泳（ＥＭＳＡ）和免疫沉淀（ＩＰ）法观察ＩＬ－６刺激作用下 ＳＹＡＴ３和 ＪＡＫ１在３种骨髓瘤细胞系（ＸＧ－７、ＫＭ－３和Ｓｋｏ－００７）中的诱导活化状态。采用ＲＴ－ＰＣＲ和 Ｗｅｓｔｅｒｎ－ｂｌｏｔ法检测这两种信号蛋白分子在以上 ３株靶细胞中的表达情况。结果 尽管ＳＴＡＴ３在３株靶细胞中都能够正常表达，但只有Ｓｋｏ－００７细胞中出现ＩＬ－６刺激作用下ＳＴＡＴ３的诱导活化。在ＸＧ－７细胞中，既没有检测到ＪＡＫ１的表达，也没有观察到ＪＡＫ１的活化。尽管ＪＡＫ１在ＫＭ－３细胞中能够正常表达，但不能被ＩＬ－６诱导激活。Ｓｋｏ－００７细胞中则同时出现 ＪＡＫ１的表达及ＩＬ－６刺激后的诱导活化。结论 ＪＡＫ１的正常表达和激活是ＩＬ－６刺激作用下ＪＡＫ／ＳＴＡＴ信号转导途径在骨髓瘤细胞中诱导活化的前提条件。
The purpose of the tyrosine protein kinase JAK1 and the transcription factor STAT3 are the two major signaling protein molecules involved in the IL-6-induced JAK / STAT signal transduction pathway. This study sought to reveal the role of JAK1 in the induction of activation by the JMK / STAT pathway. Methods The expression of SYAT3 and JAK1 in three kinds of myeloma cell lines (XG-7, KM-3 and Sko-007) under IL-6 stimulation were observed by gel electrophoresis (EMSA) and immunoprecipitation (IP) Induction of activation. RT-PCR and Western-blot were used to detect the expression of these two signaling molecules in the above three target cells. Results Although STAT3 was normally expressed in all three target cells, only STAT3-induced activation of Sko-007 cells was observed. In XG-7 cells, neither JAK1 expression nor JAK1 activation was observed. Although JAK1 is normally expressed in KM-3 cells, it can not be activated by IL-6. In Sko-007 cells, JAK1 expression and induction activation after IL-6 stimulation were also observed. Conclusion The normal expression and activation of JAK1 is a prerequisite for inducing activation of JAK / STAT signal transduction pathway in myeloma cells under the stimulation of IL-6.