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目的:探讨大鼠角膜层间植入微粒体诱导新生血管模型的制作技巧和特点。方法:9只SD大鼠手术显微镜下做角膜基质微囊袋并植入含有VEGF的缓释微粒体。术后1、3、5、7d采用裂隙灯显微镜观察角膜新生血管的生长情况。结果:大鼠角膜微囊袋内植入微粒体后1d仅见角膜缘血管网充血扩张,未见新生血管生长;3d可见毛刷状新生血管自角膜缘伸入角膜,面积为(2.23±0.59)mm2;5d新生血管垂直于角膜缘向微粒体方向束状延伸,并达到微粒体下缘部位,面积为(6.81±1.35)mm2;7d新生血管继续向前生长,达到并超过微粒体下缘,部分新生血管顶端互相吻合成袢状,面积为(8.92±1.79)mm2。其中,5d和7d新生血管面积比较无显著性差异。所有术眼均未见前房积血、角膜溃疡、微粒体脱出等并发症。结论:大鼠角膜层间植入微粒体诱导的新生血管生长稳定、无并发症、适用于定量研究。
Objective: To explore the techniques and characteristics of rat corneal interstitial microsomal-induced angiogenesis model. Methods: Nine SD rats underwent corneal microencapsulation and implantation of sustained-release microsomes containing VEGF. The growth of corneal neovascularization was observed with slit lamp microscope at 1, 3, 5 and 7 days after operation. Results: Only the corneal vascular network was inflated and expanded on the first day after implantation of microsomes in the cornea of the rat cornea. No angiogenesis was seen on the cornea of the cornea at 3 days. The corneal neovascularization reached the cornea from the corneal margin on 3d, with an area of (2.23 ± 0.59) mm2; 5d neovascularization extends perpendicularly to the direction of corneal microsomes and reaches the lower edge of microsome with an area of (6.81 ± 1.35) mm2; on the 7th day, angiogenesis continues to grow forward and beyond the lower edge of microsome, Some of the neovascular apical anastomosis 成 shaped, with an area of (8.92 ± 1.79) mm2. Among them, there was no significant difference in the area of neovascularization between 5d and 7d. All eyes were no anterior chamber hemorrhage, corneal ulcer, microsomal prolapse and other complications. Conclusion: Microscopically implanted corneal interstitial microsphere-induced neovascularization is stable and has no complications and is suitable for quantitative study.