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利用SRAP分子标记技术对新疆野核桃遗传多样性进行分析。通过筛选出的15对具有多态性的SRAP引物组合进行PCR扩增,得到新疆野核桃遗传分化系数Gst为0.1152,说明新疆野核桃的遗传变异绝大部分存在于区域内部,占总变异的88.48%;多态位点百分率为94.07%,Shannon’s信息指数I=0.4954,等位基因平均数Na=1.9454,表明新疆野核桃具有较高的遗传多样性;各区域间遗传相似系数在0.8981~0.9496之间,遗传距离在0.0553~0.1075之间,说明新疆野核桃资源间存在着丰富的遗传变异。通过聚类分析可聚为2类,进一步明确了新疆野核桃各区域之间的亲缘关系。
Genetic diversity of wild walnut in Xinjiang was analyzed by SRAP molecular marker. Fifteen pairs of SRAP primers with polymorphism were selected for PCR amplification. The genetic differentiation coefficient of Ginkgo biloba in Xinjiang was 0.1152, indicating that most of the genetic variation in Xinjiang wild walnut existed in the region, accounting for 88.48 %; The percentage of polymorphic loci was 94.07%, Shannon’s information index I = 0.4954, and the average number of alleles Na = 1.9454, which indicated that Xinjiang wild walnut had higher genetic diversity. The genetic similarity coefficient of each region was 0.8981 ~ 0.9496 Between the genetic distance of 0.0553 ~ 0.1075, indicating that there is a rich genetic variation between wild walnut resources in Xinjiang. Clustering analysis can be clustered into two categories, further clarifying the relationship between Xinjiang wild walnut regions.