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目的对新疆琐琐葡萄提取物抗流感病毒A(H1N1)亚型作用进行研究。方法1.采用狗肾传代细胞培养法和MTT法研究新疆琐琐葡萄多糖、琐琐葡萄总黄酮、琐琐葡萄总三萜对抗流感病毒A(H1N1)型作用。2.采用Read-muench法计算流感病毒A(H1N1)型在狗肾传代细胞中的半数感染量。3.通过观察细胞病变(CPE)、计算细胞病变CPE抑制率、MTT法测定药物对细胞的保护率等来评价琐琐葡萄多糖、总黄酮、总三萜的抗流感病毒效果。结果琐琐葡萄总黄酮的最大无毒作用浓度(TC0)为20μg/ml,琐琐葡萄总三萜的TC0为1500μg/ml。体外细胞试验中,琐琐葡萄黄酮、多糖提取物表现出对病毒的直接抑制作用,但是琐琐葡萄三萜提取物和利巴韦林(阳性对照)此作用不明显。体外细胞试验中,琐琐葡萄黄酮、多糖、三萜提取物均表现出对感染细胞后病毒的抑制作用,三萜的抑制效果要强于黄酮和多糖,利巴韦林的抑制作用强于黄酮、多糖、三萜。结论新疆特色植物资源—琐琐葡萄提取物抗流感病毒A(H1N1)亚型的作用是多途径的,琐琐葡萄总黄酮、琐琐葡萄多酚、琐琐葡萄三萜分别表现出对流感病毒A(H1N1)亚型感染细胞前、感染细胞后的抑制作用,以及直接抑制作用,可考虑作为抗流感药用资源进行进一步的开发利用。
Objective To study the anti-influenza virus A (H1N1) subtype of Xinjiang SuoSuo grape extract. Methods 1. The effects of influenza virus A (H1N1) type on polysaccharides of Xinjiang SuoSuoGroup, total flavone of SuoSuoGuo and total triterpenoids of SuoSuoGuo were studied by using canine kidney passage cell culture method and MTT method. 2. Read-muench method was used to calculate the influenza A (H1N1) type in dog kidney passage cells in half the amount of infection. To evaluate the anti-influenza virus effects of polysaccharides, total flavones and total triterpenoids by observing cytopathic effect (CPE), calculating CPE inhibitory rate of cytopathic effect and determining the protection rate of cells by MTT assay. Results The maximum nontoxic concentration (TC0) of the total flavones of SuoSuo grapes was 20μg / ml and that of the total triterpenes of SuoSuo grapes was 1500μg / ml. In in vitro experiments, the effects of flavones and polysaccharides of Superoxide showed a direct inhibitory effect on the virus, but triterpenoid extract of SuoSuo grape and ribavirin (positive control) had no obvious effect. In vitro cell tests, the flavones, polysaccharides and triterpene extracts of SuoSuoLing showed the inhibitory effect on virus after infected cells, the inhibitory effect of triterpenoids was stronger than that of flavonoids and polysaccharides, the inhibitory effect of ribavirin was stronger than flavonoids, Polysaccharides, triterpenes. Conclusion The function of anti-influenza virus A (H1N1) subtype of the extract of SuoSuo grape in Xinjiang is multi-pathways. Triterpenoid total flavone, SuoSuo grape polyphenol and SuoSuo grape triterpenes respectively show the anti-influenza virus Influenza A (H1N1) subtype infected cells before and after infection, inhibition, and direct inhibition can be considered as anti-influenza drug resources for further development and utilization.